Family-based genetic risk prediction of multifactorial disease
© Ruderfer et al.; licensee BioMed Central Ltd. 2010
Received: 22 July 2009
Accepted: 15 January 2010
Published: 15 January 2010
Genome-wide association studies have detected dozens of variants underlying complex diseases, although it is uncertain how often these discoveries will translate into clinically useful predictors. Here, to improve genetic risk prediction, we consider including phenotypic and genotypic information from related individuals. We develop and evaluate a family-based liability-threshold prediction model and apply it to a simulation of known Crohn's disease risk variants. We show that genotypes of a relative of known phenotype can be informative for an individual's disease risk, over and above the same locus genotyped in the individual. This approach can lead to better-calibrated estimates of disease risk, although the overall benefit for prediction is typically only very modest.
Although whole-genome association studies have detected dozens of common variants for a broad range of complex diseases, and are likely to detect many more, the total variance explained by the known variants is typically modest [1, 2]. As such, realising the goals of accurate genetic risk prediction and the subsequent opportunities of personalised medicine remains difficult [3, 4]. Indeed, it has often been noted that family history alone will perform substantially better as a predictor of risk, compared to genotype data for known risk variants . It is true that a positive family history will likely remain an important factor in prediction for the many complex diseases with substantial heritabilties and shared familial environmental components. (A caveat is that family history information might sometimes not be straightforwardly available, for example, for phenotypes such as response to a particular drug treatment.) However, analogous to clinical genetic testing for Mendelian disease, it is plausible that in many cases a positive family history will itself be a motivating factor for pursuing a genetic test. For example, an individual whose older sibling developed a particular disease might be particularly concerned with their own personal risk, which they assume will be higher than average. In this context, in which a genetic test is sought because a first-degree relative has disease, we developed a family-based model for risk prediction incorporating genotype data from both the index individual and a relative of known phenotype. As such, we do not ask "how well do SNPs predict disease compared to family history", but rather, "how well do SNPs predict disease given a positive family history, and to what extent does including genotype data from the affected relatives help?".
Information from relatives of known phenotype
For diseases with polygenic and shared environmental components of risk, the genotype of a relative of known phenotype can be informative for an individual's disease risk, over and above the individual's own genotype at that locus. Below, the term genotype here refers to both single and multi-locus genotypes, unless explicitly stated. We assume that genotypes at the locus or loci under consideration only account for a proportion of the total familial covariance, meaning that unmeasured residual polygenic and/or shared environmental factors still exist, as would be expected for a complex disease.
then if E(b1) > 0, for example, E(b2) will no longer equal zero. In fact, in this case, E(b2) < 0 meaning that the sibling's genotype is informative for the index's disease risk, in the opposite direction compared to b1.
Why is the sibling genotype conditional on index genotype and sibling phenotype informative for index disease risk? For a given risk locus, if the sibling is affected but has a low-risk genotype, this implies that the index is at higher risk than if the affected sibling has a high-risk genotype, conditional on the index's own genotype at that locus. In this scenario, the affected sibling's genotype acts as a surrogate for all other unmeasured risk factors: if the sibling has the low-risk genotype but still is affected, he or she is likely to have a higher rate of other, unobserved risk factors, either genetic or environmental. To the extent that these unobserved risk factors are shared among siblings, the affected sibling's genotype will therefore act as a surrogate for the index's unobserved risks. This is analogous to the epidemiological phenomenon of selection bias, in which an association arises due to shared but unmeasured factors.
In general, a lower genetic load of known risk variants in an affected relative will tend to increase the index's risk of disease, over and above the level of risk predicted by the index's own genotype. For the index, a higher genetic load still leads, as usual, to a higher predicted risk. (Note that if we did not know the index genotype, the affected relative's genotype would act as a surrogate for it. In this case, a higher load of known risk variants in the affected relative would predict a higher, not lower, risk in the index. Unless the affected relative is an MZ twin, prediction would naturally be worse than if we knew the actual index genotype.) In the rest of this report, we applied this observation to the problem of genetic risk prediction, asking whether the inclusion of genotypes from a relative of known phenotype can improve the accuracy of prediction.
Prediction model incorporating family information
Here we introduce a model in which the relative of known phenotype is an affected sibling; the basic approach can be easily extended to other and multiple relative types. Specifically, we wish to predict disease risk for the index individual, conditional on a) their multilocus genotype at V known disease variants, b) their affected sibling's disease state and c) additionally including the affected sibling's multilocus genotype.
where P(D I , D S |G I , G S ) and are directly obtained from the multivariate normal cumulative distribution function, assuming a liability-threshold model for disease risk.
Simulation study of Crohn's disease variants
Crohn's disease model specification
In all cases, we set the polygenic variance components = 0.7, = 0.2 and = 0.1, which implies a risk to individuals with at least one affected sibling of 0.11, and therefore, a sibling relative risk of 28.6 . Note that the performance of the family model depends on the residual sibling correlation and not just the individual values of values of and (i.e. all pairs of values that yield the same implied sibling correlation will show identical performance).
For the unselected population we simulated 500,000 nuclear families, each with two siblings. For the family-history positive population, we simulated 100,000. Fewer replicates were required due to the much higher baseline rate for D I in this population.
Results and discussion
Single locus example
To illustrate the approach, we analytically calculated the expected risk under a variety of models, based on information from a single locus - rs2188962, one of the Crohn's disease (CD) loci identified in a recent meta-analysis , setting the genotypic relative risk (GRR) to 1.25 and the risk allele frequency (RAF) to 0.425. Prevalence, additive polygenic and shared environmental components of variance were set to approximate known values for CD, as described above. Figure 1 shows the predicted disease risks under five models:
no information, P(D I );
conditional on index genotype, P(D I |G I );
conditional on having an affected sibling status alone, P(D I |D S );
as above, including index genotype, P(D I |G I , D S );
Conditional on index genotype, the affected sibling's genotype further stratifies risk, but with the low-risk genotype predicting increased risk for the index. Values of P(D I |G I ) only range around P(D I ), from 0.32% to 0.52% for the low-risk to high-risk homozygotes, whereas P(D I |G I , G S , D S ) shows a much greater range around P(D I |D S ), from 8.9% to 14.6%. The predicted risks shown here were reproduced by simulating data under this model and calculating the proportion of index cases for each configuration (data not shown).
Crohn's disease simulation
We next performed a simulation as described above that included all 30 CD variants reported in Barrett et al  that collectively account for 6.4% of the total variance (calculated assuming a liability-threshold model and assuming additivity across loci on the scale of liability). We first simulated a simple unascertained sample of nuclear families, each with two siblings (i.e. D S will only be affected at the usual population prevalence). Second, we used rejection sampling to simulate an ascertained sample in which at least one sibling was affected (D S is always affected). For each simulated family, we calculated the risk for the index being affected, D I using the methods described above.
Crohn's disease simulation results
P(D I |G I )
P(D I |G I , D S )
P(D I |G I , G S , D S )
Selected population (affected sibling)
P(D I |G I , D S )
P(D I |G I , G S , D S )
We first describe results for the general population, in which nuclear families were generated without any ascertainment on disease. As expected, compared to the basic model P(D I |G I ), the inclusion of a sibling phenotype D S (which might be affected or unaffected) improved both risk prediction for the index, particularly as indexed by R2 (0.054 to 0.085). The enrichment of cases in the highest-ranked 1% (T1) more than doubled (7.39 to 15.9). In this population, however the addition of the sibling's genotypes G S added only marginal benefit in terms of AUC and R2, and no benefit for the T metrics.
In the second population, we ascertained for a positive family history (i.e. D S is always affected). Of note, compared to the unselected population, the AUC and R2 metrics are considerably lower in this high-risk population, whereas the T metrics are substantially higher (largely reflecting the high sibling relative risk for this disease). That the discriminative performance of a test may vary depending on the characteristics of the population it is deployed in may have important implications for the generalizability of studies that claim a certain AUC, which is not an invariant property of the test alone but depends on the context in which it is used.
In terms of discrimination, the basic P(D I |G I ) model as expected yields near identical results compared to P(D I |G I , D S ), as all siblings are affected in this population; we therefore omit this model here. However, the absolute values of predicted risk based on P(D I |G I ) will be very poorly calibrated, as this model ignores the presence of a positive family history. For example, for individuals with a predicted risk of 0.1 ± 0.01 from the P(D I |G I , D S ) model, we observed a rate of 0.099 cases in the simulated data. However, based on P(D I |G I ), these same individuals had a mean predicted risk of only 0.0037. In other words, by not conditioning on known affected sibling status, the prediction model will dramatically under-estimate the absolute risks.
Finally, we considered whether adding sibling genotypes improved prediction in this family-history positive population. We observed negligible improvement in AUC (1.03-fold increase) but a larger increase for R2 (1.33-fold, 0.042 to 0.056). There were also increases in the already-large T metrics. As expected, the benefit derived from including sibling genotypes is larger in the ascertained population, as for a relatively rare but highly familial disease, affected siblings will be more informative than unaffected siblings. In the family-history positive population, adding affected sibling genotypes offers some advantage, although likely not enough to ever fundamentally change the discriminative utility of a test.
Including affected sibling genotypes can improve the calibration of predicted risks somewhat and lead to a greater stratification of risk, as apparent in Figure 1. We can quantify the risk stratification depicted in Figure 1 in terms of a metric δ. Comparing two sets of predicted risks, we define δ as the expected change in risk, calculated as ∑ i |P i -Q i |/N of N total individuals, P i is the probability of disease in the individual before the test and Q i is the probability afterwards. This is one way of characterizing the personal impact of a test: the expected change in estimated risk pre- versus post-test. In the family-history positive population, δ for P(D I |G I , D S ) is 0.035; the incremental δ going from the risks estimated based on P(D I |G I , D S ) to P(D I |G I , G S , D S ) is 0.02. In other words, updating one's risk based on an affected sibling's genotype would be expected to change one's predicted risk 57% (0.02/0.035) as much as the initial test (in the unselected population, this value is 50%).
Including additional and/or unaffected family members
We also considered models in which additional affected family members are included in the model: for example, individuals in multiplex families with an affected sibling and an affected parent, or two affected siblings. In general, we do see improvement from incorporating the genotypes of these additional affected relatives, although there tends to be a diminishing return (data not shown).
In practice, for most diseases, being of relatively low frequency (e.g. under 10%), only affected relatives will contribute information, compared to relatives known to be disease-free. In addition, determination that an individual is disease-free with respect to life-time risk might be difficult.
One caveat is that if the known variants used in the test themselves account for the entire familial covariance, then genotypes from phenotyped relatives will not contribute any additional information. This is unlikely to be the case in the foreseeable future for most diseases, however; it would imply that we have already maximized the potential of genetic risk prediction.
For this work we have assumed a particular model for risk, additivity on the scale of liability, which in practice approximates a multiplicative model on the scale of risk. This implies that the same risk ratio will correspond to a larger absolute risk difference if there is a higher baseline risk: for example, 1% versus 2% and 5% versus 10% both imply risk ratios of 2, but varying absolute risk differences. This effect is evident in Figure 1, in which genotype leads to a greater stratification of absolute risk in individuals with an affected sibling. Whether or not the implied penetrances for individuals with a positive family history actually follow this model is a question that ultimately should be empirically addressed, to indicate the adequacy of the risk model. However, this does not alter the qualitative principle outlined here that relatives' genotypes and phenotypes are informative for an individual's disease risk.
We observed that the genotypes of relatives of known phenotype are informative for an individual's risk, independent of the same risk variants measured in the index individual. We sought to determine whether this phenomenon could be of use in the context of genetic disease risk prediction. We described and evaluated a prediction model for individuals with one or more affected first-degree relatives. Our model has the key feature of incorporating genotype information from relatives to improve the accuracy of prediction. The basic insight - that affected relatives' genotypes are informative about an individual's risk for a multifactorial, polygenic disease - is not confined to the particular analytic approach presented here and could be used with other prediction methodologies. In this work, we focused on the additive effects of confirmed disease alleles, although others have incorporated other sources of information, including non-genetic risk factors  and interactions between risk factors . To the extent that such risk factors are shared between relatives, the approach outlined here to include information from affected relatives could also be applied in these other contexts. Methodologically, we used a liability threshold model. Others have developed prediction models using logistic regression , optimal ROC curves , Bayesian networks  and support vector machines , using diverse criteria to evaluate performance in terms of, for example, discrimination, calibration and reclassification . Again, information from affected relatives could in theory be included using any of these approaches. In fact, our approach is conceptually similar to methods in livestock genetics and animal breeding that use genetic marker data for prediction, using all the data and taking into account familial relationships in complex pedigrees . However, in the context of human disease risk prediction, our simulations suggest that, in most cases, only incremental improvements are to be expected, meaning it is unlikely that the overall applicability of a test will be fundamentally altered.
area under the curve
genotypic relative risk
minor allele frequency
risk allele frequency
receiver operating characteristic
single nucleotide polymorphism
This work was supported by a NARSAD Young Investigator Award (SMP). We thank Colm O'Dushlaine, James Wilkins, Ben Neale and Mark Daly for helpful discussion.
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