Circulating tumor cells and DNA as liquid biopsies
© BioMed Central Ltd 2013
Published: 23 August 2013
For cancer patients, the current approach to prognosis relies on clinicopathological staging, but usually this provides little information about the individual response to treatment. Therefore, there is a tremendous need for protein and genetic biomarkers with predictive and prognostic information. As biomarkers are identified, the serial monitoring of tumor genotypes, which are instable and prone to changes under selection pressure, is becoming increasingly possible. To this end, circulating tumor cells (CTCs) or circulating tumor DNA (ctDNA) shed from primary and metastatic cancers may allow the non-invasive analysis of the evolution of tumor genomes during treatment and disease progression through 'liquid biopsies'. Here we review recent progress in the identification of CTCs among thousands of other cells in the blood and new high-resolution approaches, including recent microfluidic platforms, for dissecting the genomes of CTCs and obtaining functional data. We also discuss new ctDNA-based approaches, which may become a powerful alternative to CTC analysis. Together, these approaches provide novel biological insights into the process of metastasis and may elucidate signaling pathways involved in cell invasiveness and metastatic competence. In medicine these liquid biopsies may emerge to be powerful predictive and prognostic biomarkers and could therefore be instrumental for areas such as precision or personalized medicine.
Monitoring cancer therapy
Improvement in the clinical outcome of many cancer types is likely to be achieved by giving patients a drug tailored to the genetic makeup of their tumor. One of the best documented examples is the breast cancer drug trastuzumab (Herceptin), a monoclonal antibody that blocks the human epidermal growth factor receptor 2 (HER2) and is given to breast cancer patients whose tumors overexpress HER2 . The recent surge in high-throughput sequencing of cancer genomes is delivering cheaper and more accurate tumor genome information [2–5], and it can be anticipated that more predictive biomarkers will be identified and that patients will increasingly be treated by focusing on the genetic architecture of their particular tumor rather than on the tumor's location or histological features.
However, cancer genomes are unstable and prone to changes under selection pressures such as the application of therapies. Thus, molecularly targeted cancer therapies require serial monitoring of the tumor genome's makeup to ensure that a given therapy is still 'hitting the target' or to detect whether new predictive biomarkers are emerging. However, serial biopsies of metastatic lesions cannot usually be performed because of their invasive nature. Furthermore, they frequently yield only sparse quantities of cytological material and therefore provide only limited information about the genetic content of cells. In fact, the inability to obtain adequate material for serial monitoring of tumor genotypes has been a major barrier to translating laboratory findings into therapy.
Methods for rapid, cost-effective, and non-invasive monitoring of tumor genomes and for identification of potential biomarkers at various time points during a disease course are needed. To this end, circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) offer a unique opportunity for serially monitoring tumor genomes in a non-invasive manner. As CTCs and ctDNAs are potential surrogates for the tumor itself, they are often referred to as 'liquid biopsy' [6–10].
CTCs are shed into the bloodstream from primary and metastatic tumor deposits. First observed in 1869 by the Australian physician Thomas Ashworth , the potential value of CTCs was not realized until the end of the 1990s. An initial focus of research when it began in earnest in the early 1990s was the rare tumor cells found in the bone marrow at the time of diagnosis, long before metastasis was evident [8, 9]. In contrast to CTCs (tumor cells in the circulation), these disseminated tumor cells may remain in a dormant state for many years at a site in the body, such as in the bone marrow, and may give rise to metastases years after complete resection of the primary tumor. For example, in patients with breast cancer, several studies suggested that the presence of disseminated tumor cells in bone marrow is associated with a poorer prognosis [12, 13]. However, frequent bone marrow biopsies are not practical, and therefore interest has shifted to CTCs, which can be obtained by a simple blood collection.
Although the isolation and analysis of CTC hold great promise for the early detection of invasive cancer and the management of advanced disease, technological hurdles such as finding them among millions of normal hematogenous cells have limited their broad clinical utility. Here we review recent developments in CTC capture and analysis tools, CTC monitoring strategies, and insights into their biology. In addition, we cover progress in ctDNA analysis, which may become a complementary or alternative liquid biopsy to CTCs.
CTC selection methods
A major hurdle in CTC analysis is that CTCs constitute as few as 1 cell per 1 × 109 normal blood cells in patients with metastatic cancer, and therefore it is difficult to identify and isolate them . As a consequence, multiple CTC selection and capture approaches have been developed and recently extensively reviewed [6, 9, 10, 16, 17]. We refer the reader to these references for a complete overview of CTC selection technologies. Here, we focus only on some of the more commonly used CTC selection methods and on some recent microfluidics technologies, which were not covered in the aforementioned reviews.
The simplest CTC selection method is probably size-based membrane filters. Such filters enrich cells larger than a certain diameter based on the assumption that CTCs are larger than blood cells [18, 19]. However, a recent study reported a considerable size overlap between CTCs and leukocytes , so that size-based filter systems probably miss a proportion of CTCs.
At present the most widespread CTC detector is the CellSearch system (Veridex). This is a semi-automated system that enriches for cells expressing epithelial-cell adhesion molecules (EpCAMs) but lacking the leukocyte-specific molecule CD45. Cells are further immunostained with fluorescent-labeled anti-keratin antibodies identifying, among others, cytokeratin (CK)8, CK18, and CK19 [14, 21, 22]. Using the CellSearch system, CTCs were found in the peripheral blood of patients with all major carcinomas, but not in participants without malignant diseases . With this system, baseline and follow-up CTC levels were reported to be strong predictors for progression-free and overall survival, which resulted in US Food and Drug Administration approval for monitoring patients with metastatic breast, prostate, and colorectal cancer .
To optimize the capture of CTCs, several microfluidic platforms have been developed (reviewed by Pratt et al. ). One is the CTC-chip, in which blood flows past EpCAM-coated microposts under precisely controlled laminar-flow conditions . However, the difficulties in isolating these cells in this non-transparent three-dimensional array of microposts limited the options for subsequent single CTC analyses. Therefore, the same group developed this chip further, and instead of microposts the advanced chip uses surface ridges or herringbone grooves in the ceiling of the channel (it is thus dubbed the herringbone- or HBCTC-Chip). The grooves generate transverse flow, inducing microvortices, which direct cells toward the EpCAM-coated walls of the device [25, 26]. Advantages include the use of transparent wells and less complex three-dimensional structures, facilitating analysis of captured cells.
Another microfluidic device for enhanced CTC capture is the micro-Hall detector, which detects the magnetic moments of cells in-flow after immunolabeling with magnetic nanoparticles. The detection of CTCs was achieved using a panel of immuno-magnetic nanoparticles against four markers, EpCAM, HER2/neu, epidermal growth factor receptor (EGFR), and mucin-1 (MUC1) .
However, devices depending on EpCAM for CTC capture have the drawback that they miss EpCAM-negative CTCs, such as cells that have undergone an epithelial-to-mesenchymal transition (EMT), a process thought to facilitate the dissemination of tumor cells into the surrounding tissue and circulation . Furthermore, the heterogeneity of EpCAM expression on the surface of CTCs can cause variation in the ability to detect them . In addition, EpCAM methods cannot detect non-epithelial cancers, such as sarcomas.
In part, these limitations can be addressed by using cocktails of antibodies or by negative filtration, which uses antibodies to remove blood cells from a sample and leaves behind tumor cells. A new, more sophisticated approach to distinguish epithelial from mesenchymal cancer cells used RNA in situ hybridization (RNA-ISH) to differentially stain cells according to the expression levels of epithelial and mesenchymal genes . Another CTC capture platform, the CTC-iChip, is capable of isolating both EpCAM+ and EpCAM- CTCs using a series of steps: debulking (cytoreduction to remove red blood cells, platelets, and plasma proteins, so that only nucleated cells, white blood cells, and CTCs are retained); inertial focusing (a microscale hydrodynamic phenomenon used to position or focus randomly distributed cells - for example, in a single central stream); and magnetic separation (separation of magnetically labeled cells from unlabeled cells). These steps allow strategies for CTC isolation that is either dependent on or independent of the tumor membrane epitope, making the CTC-iChip applicable to virtually all cancers, that is, to epithelial and non-epithelial cancers .
However, these new CTC microfluidic devices have not been validated to a comparable level as the CellSearch system with regard to their specificity, reproducibility, and clinical relevance so far, so it will be of interest to see which make it into the clinic. In any case, all separation strategies will probably miss some cancer cells, and a particular challenge will be to answer the question of whether the cells that are collected are the ones that can seed new tumors. To answer essential questions about CTC biology such as this, analysis tools as outlined below are needed.
Currently the main clinical CTC application consists of enumeration of putative CTCs without further analyses. However, counting cells barely makes use of the potential information residing in CTCs and does not reflect how oncologists want to use CTCs. In general, there are various approaches to studying CTCs and to analyzing their protein, RNA, and DNA content [9, 10].
One protein-based analysis is the EPISPOT (EPithelial ImmunoSPOT) assay, which captures proteins secreted from CTCs during a 24 to 48 hour culture on a membrane coated with antibodies. This assay can distinguish between apoptotic and viable CTCs and has the potential to identify and differentiate between different proteins within CTCs (CTC protein fingerprinting) [6, 30].
Another protein analysis approach uses quantitative immunofluorescence to simultaneously visualize differently labeled targets within CTCs. For example, Miyamoto et al.  first selected prostate cancer-specific candidate gene products for which reliable antibodies were available: prostate-specific antigen (PSA; KLK3) and prostate-specific membrane antigen (PSMA; FOLH1). They then used antibodies against PSA and PSMA to distinguish CTCs from men with prostate cancer with activated and inactivated androgen receptor (AR) signaling using a quantitative immunofluorescence assay (see below).
There are also various ways to study RNA expression in CTCs. As mentioned before, one method is the quantifiable, dual-colorimetric RNA-ISH assay developed by Yu et al. , which uses the expression of seven pooled epithelial and three mesenchymal transcripts. By direct visualization of the hybridization pattern within cells, CTCs with epithelial and mesenchymal composition could be identified. Alternatively, RNA can be extracted and sequenced. For example, Stott et al.  detected the prostate cancer-specific TMPRSS2-ERG fusion in RNA extracted from CTCs from patients with metastatic prostate cancer. Microfluidic-based single cell expression analysis was reported in individual CTCs. This high-dimensional gene expression measurement allowed transcriptional profiling of 87 cancer-associated and reference genes on a cell-by-cell basis .
Recently it has also been shown that CTC lines can be developed and kept in long-term culture [33, 34]. Such CTC lines can be used for further CTC characterization and to investigate functional CTC properties, such as invasiveness or preferential sites for metastases when xenografted into nude mice.
Several recent studies have suggested that high-resolution single-cell DNA sequencing is feasible [37, 40–45]. However, all of these strategies depend on an initial WGA step, because a single diploid cell contains only 6.6 pg of DNA, whereas micrograms of DNA are required for sequencing (Figure 2). As a consequence, single-cell sequencing is prone to artifacts, which may be introduced during either amplification or sequencing. We have recently published the first study that analyzed genome-wide copy number changes and mutations in a panel of genes by next-generation sequencing (NGS) in single CTCs . In our previous papers we explained why single-cell CTC sequencing differs from sequencing of other single cells [37, 46]. First, somatic mutations in tumor cells include many changes at the nucleotide level and copy number of DNA segments . However, the reliable and simultaneous detection of both copy number changes and mutations at the nucleotide level cannot be achieved by most recently published single-cell approaches [40, 42–44]. A recent amplification method reported the genome-wide detection of single-nucleotide and copy number variations in single human cells [41, 45]. This new WGA method, 'multiple annealing and looping-based amplification cycles' (MALBAC), uses five initial quasi-linear pre-amplification cycles, which are intended to reduce the bias often observed with nonlinear amplification. This is then followed by exponential amplification cycles. However, this new method has not yet been applied to CTCs, so it remains unclear whether it can be combined with any of the CTC capture procedures. Second, as CTCs are extremely rare, usually only a very limited number of cells are available for analysis. Hence, strategies such as calling mutations only if they are observed in a specified number of cells, which have been used by some single cell sequencing approaches [40, 44], are not applicable to CTCs. Third, isolation of CTCs from millions of normal cells is a much more complex procedure than isolation of cells from a primary tumor or from another cell population. Fourth, no suitable material for comparison of CTC results is available. This is because CTCs may recur years after initial diagnosis of the primary tumor and may have acquired multiple, new changes since then. Fifth, CTCs may be released from various metastatic sites and their origin cannot usually be traced. Finally, CTCs have been reported to be heterogeneous [47–49] and may therefore show tremendous cell-to-cell variability.
Nevertheless, the importance of a molecular characterization of CTCs is highlighted by the fact that in our study  the CellSearch system selected epithelial and CD45-negative cells, in which we did not find tumor-specific mutations previously identified in the respective primary tumors and which had each a balanced copy number profile. Instead of CTCs these cells could be circulating epithelial cells, which had recently been described in patients with benign colon diseases . Thus, it is possible that not all epithelial, CD45-negative cells are CTCs.
Disease monitoring with CTCs
One of the most attractive goals of CTC analysis is disease monitoring. Indeed, even the presence of CTCs in peripheral blood is a prognostic biomarker and can be measured to track therapeutic response in patients with cancer [14, 21, 23, 51–53]. However, monitoring has gone far beyond simple CTC counting. As cancer can develop resistance against a given therapy and may then recur or spread, the timely identification of secondary mutations is of utmost importance, so that patients who are unlikely to benefit from continuing the initial therapy are spared the side effects and the loss of time that could have been spent in pursuing more active agents.
For example, patients with non-small-cell lung cancer and somatic activating mutations in the EGFR gene are likely to benefit from treatment with selective EGFR kinase inhibitors. However, secondary EGFR mutations that inhibit the binding of tyrosine inhibitors and cause resistance to such drugs may emerge . Using the CTC-chip, CTCs from patients with metastatic non-small-cell lung cancer were subjected to serial EGFR mutation analysis. Indeed, by monitoring CTCs the acquisition of the recurrent T790M-EGFR drug resistance mutation became evident and coincided with the development of clinically refractory disease .
In contrast to this approach, which focused on mutation analysis for a specific, previously known resistance marker , genome-wide analysis strategies should, in theory, capture all possible mechanisms of resistance. For example, the results of our CTC analyses using array-CGH and NGS  suggested that we can indeed elucidate relevant changes in the tumor genome that had either not been present or not been observed at the time of initial diagnosis. Analysis of the genomes of the primary tumor and metastasis of one of our patients did not reveal changes that would have made her eligible for enrolment in a trial with a CDK inhibitor such as alvocidib. Yet the CTCs obtained 34 and 24 months after diagnosis of the primary tumor and liver metastasis, respectively, revealed a high level of amplification of CDK8, which had not been noted in the previous analyses . Therefore, this amplification may be a viable target for CDK inhibitors, which are currently in clinical trials [56–58].
Miyamoto et al.  used the HBCTC-Chip and analyzed CTCs with the aforementioned PSA and PSMA quantitative immunofluorescence assay. PSA is upregulated following androgen receptor (AR) activation and PSMA is upregulated following AR suppression, so that PSA+ PSMA- CTCs indicate activated AR whereas CTCs with a reverse pattern, PSA- PSMA+, indicate suppressed AR. In untreated patients, single-CTC immunofluorescence analysis revealed predominantly the expected PSA+ PSMA- signatures. Initiation of first-line androgen deprivation therapy switched this pattern to PSA- PSMA+. The presence of a mixed PSA+ PSMA+ pattern or CTCs with PSA+ PSMA- despite treatment was associated with an adverse treatment outcome. These data suggested that CTCs may help to guide therapy in prostate cancer .
In summary, CTC characterization might be a useful biomarker for treatment response and for monitoring changes in tumor genomes.
The other current area of CTC research addresses their biology to learn more about the process of metastasis . The mechanisms that implicate CTCs in metastatic disease remain elusive, and a particular challenge is to answer the question of whether the cells that are collected with the aforementioned approaches have the potential to seed new tumors. To this end, mouse models are instrumental . For example, mouse models revealed that metastasis is not a unidirectional process but a multidirectional one in which cancer cells do not seed only distant sites, but also the primary tumor itself. This latter process has been termed 'self-seeding' [60, 61].
It has been hypothesized that CTCs include putative precursors that initiate distal metastases. However, the existence and phenotype of such putative metastasis-initiating cells remains elusive.
Using an endogenous mouse pancreatic cancer model, single-molecule RNA sequencing from CTCs identified enriched expression of Wnt2. Indeed, expression of WNT2 in human pancreatic cancer cells was shown to increase metastatic propensity in vivo . Another study used a xenograft assay to demonstrate that primary human luminal breast cancer CTCs contain cells that give rise to metastases in mice in various organs. These metastasis-competent CTC populations expressed EpCAM, CD44, CD47, and MET, and in a small cohort of patients with metastases it could be shown that the presence of CTCs with such an expression profile was correlated with lower overall survival and increased number of metastatic sites . Thus, mouse models contribute to the identification of functional circulating metastasis-competent CTCs, and their molecular analysis has great potential to identify candidate therapeutic targets to prevent the distal spread of cancer.
Another exciting topic is the role of EMT in tumor metastasis. Yu et al.  measured the expression of mesenchymal and epithelial markers in CTCs from breast cancer patients. Serial CTC monitoring suggested an association of mesenchymal CTCs with disease progression. In one patient, reversible shifts between mesenchymal and epithelial cell fates were associated with response to therapy and disease progression . Another recent study focused specifically on EpCAM- CTCs . In patients with breast cancer, cell identification was then based on protein detection by immunofluorescence or FISH to detect CTCs overexpressing EGFR, HPSE, a potent tumorigenic, angiogenic, and prometastatic molecule, and ALDH1, a tumor-initiating stem cell marker. The authors  also developed CTC lines from some of these patients and identified a potential expression signature of brain metastasis, which was, in addition to the overexpressed EGFR and HPSE, characterized by high expression of HER2 and Notch1 .
In summary, studying CTC biology has the potential to elucidate the role of EMT and to discover processes instrumental for metastasis.
ctDNA as an alternative to CTC analysis
Differences between CTC and ctDNA analyses
Special instrumentation for cell identification needed
None, simple blood collection
Isolation of CTC or ctDNA
Complex CTC isolation out of thousands of cells and complex single-cell transfer for further processing
No isolation of ctDNA required; instead, standard preparation of plasma DNA
WGA required for DNA analysis
Information on heterogeneity and clonality
Yes, if enough CTCs are captured and successfully analyzed
No, results represent an average from all cells shedding tumor DNA into the circulation
Dependence on EpCAM markers
Yes, for most CTC capture systems, such as the CellSearch system. EpCAM-independent CTC capture systems exist but await validation in clinical studies
Independent of any marker
Applicability for basic metastasis research
Instrumental, as it enables cell-by-cell analyses, generation of cell lines and analyses in animal models
Provides only a snapshot of the current status of the tumor genome
Applicability for diagnostic or monitoring purposes
Established for CTC enumeration; advancements will depend on improvements in CTC capturing, analysis tools and associated costs
Appears to be very attractive because of the simplicity of obtaining plasma DNA; needs to be determined in clinical studies
So far, recent ctDNA publications have focused on cancer patients with very advanced diseases and therefore very high concentrations of ctDNA [69, 70, 74, 76–82]. Detailed experiences with early stage cancer and low concentrations of ctDNA are lacking. Furthermore, normal DNA always dilutes the ctDNA (Figure 3), which may be aggravated during inflammation and injury when very high amounts of normal DNA are released into the circulation. However, receiver operating characteristic analyses suggested that even tumor DNA concentrations at very low levels should be detectable in the circulation of patients with cancer [78, 79]. If this can be verified in appropriate clinical studies, ctDNA appears to be attractive as a diagnostic tool because of the ease with which it can be obtained. In contrast, CTCs may in addition provide more insights into tumor biology (Table 1). However, the sensitivity of CTC versus plasma DNA as predictive and prognostic biomarkers still needs to be explored in detail in future studies.
Conclusions and future perspectives
Both CTCs and ctDNA provide snapshots of genomic alterations in primary tumors and metastases at various stages during the course of disease. Recent years have witnessed tremendous progress in the identification, capturing, and analysis of CTCs. The advantages of CTC analyses include that - provided they were selected with highly specific approaches as discussed above - they represent a pure tumor cell population. Together with new WGA approaches and NGS, these analyses mean that unique insights into clonal heterogeneity and evolution at various disease stages can be obtained. CTCs provide new options to study metastasis and to monitor the evolution of tumor genomes with high resolution. As CTCs are very rare cells, capturing them depends on rather sophisticated equipment, which is a limitation. In contrast, plasma DNA is easily obtained; however, tumor DNA fragments are diluted with various amounts of DNA from normal cells, which may hamper analysis.
Most CTC and ctDNA studies were done with patients with metastatic disease. At present, there are not enough data available to judge whether these CTC and ctDNA analyses are sensitive enough to detect a person's initial tumor early in its development. Furthermore, it is unknown whether they are suitable for screening purposes in individuals with an increased risk of developing a tumor.
However, despite the remarkable progress in recent years, several challenges remain. Better CTC detectors recognizing both EpCAM+ and EpCAM- cells at high speed with confidence are needed for clinical use. A current problem of all CTC detectors is the secure and automated transfer of single CTCs for further processing. At present, this is usually done by manual micromanipulation approaches, which require expertise and skilled personnel and which can be time consuming. This may change in the near future, as automated cell sorting and recovery devices are becoming available. For example, the DepArray system uses a non-uniform electric field, which can be used to move cells and to eventually recover cell populations in an automated way. First reports describing the isolation of a pure CTC population with this device and their subsequent comprehensive analyses were indeed promising [83, 84].
Furthermore, technologies isolating sufficient numbers of living CTCs, which can be expanded in culture or directly used for functional studies, are needed. To this end, appropriate culturing conditions for both epithelial and mesenchymal CTCs need to be established. An important question is whether the cells we are capturing are the seeds for new tumors. To this end, the involvement of the EMT process or of stem cell markers in CTCs needs to be further elucidated. If the details of these cells reveal how human cancers spread, they may offer new treatment options for stopping it at an early stage. At present, the characterization of the phenotype of metastasis-initiating cells among CTCs is still in its early infancy . Identification of such precursors may identify cellular pathways contributing to both the blood-borne dissemination of cancer and processes involved in metastatic colonization.
The technologies we describe here represent fundamental steps towards the identification of predictive and prognostic biomarkers. There is no doubt that they will evolve to new non-invasive diagnostic tests that are amenable to routine clinical use in terms of sensitivity and cost. Accordingly, they will change the management of patients with cancer as they will allow the non-invasive monitoring of tumor genomes, which presents another step towards personalized medicine.
circulating tumor cell
circulating tumor DNA
epidermal growth factor receptor
epithelial cell adhesion molecule
fluorescence in situ hybridization
human epidermal growth factor receptor 2
in situ hybridization
prostate-specific membrane antigen
We thank Maria Langer-Winter for editing the manuscript. Funding in our laboratory was provided by the Austrian Science Fund (FWF) (grant numbers P20338, P23284 and W 1226-B18, DKplus Metabolic and Cardiovascular Disease), and the Oesterreichische Nationalbank (15093).
- Spector NL, Blackwell KL: Understanding the mechanisms behind trastuzumab therapy for human epidermal growth factor receptor 2-positive breast cancer. J Clin Oncol. 2009, 27: 5838-5847. 10.1200/JCO.2009.22.1507.View ArticlePubMedGoogle Scholar
- McLeod HL: Cancer pharmacogenomics: early promise, but concerted effort needed. Science. 2013, 339: 1563-1566. 10.1126/science.1234139.View ArticlePubMedPubMed CentralGoogle Scholar
- Dancey JE, Bedard PL, Onetto N, Hudson TJ: The genetic basis for cancer treatment decisions. Cell. 2012, 148: 409-420. 10.1016/j.cell.2012.01.014.View ArticlePubMedGoogle Scholar
- Stratton MR: Exploring the genomes of cancer cells: progress and promise. Science. 2011, 331: 1553-1558. 10.1126/science.1204040.View ArticlePubMedGoogle Scholar
- Vogelstein B, Papadopoulos N, Velculescu VE, Zhou S, Diaz LA, Kinzler KW: Cancer genome landscapes. Science. 2013, 339: 1546-1558. 10.1126/science.1235122.View ArticlePubMedPubMed CentralGoogle Scholar
- Alix-Panabieres C, Pantel K: Circulating tumor cells: liquid biopsy of cancer. Clin Chem. 2013, 59: 110-118. 10.1373/clinchem.2012.194258.View ArticlePubMedGoogle Scholar
- Maheswaran S, Haber DA: Circulating tumor cells: a window into cancer biology and metastasis. Curr Opin Genet Dev. 2010, 20: 96-99. 10.1016/j.gde.2009.12.002.View ArticlePubMedPubMed CentralGoogle Scholar
- Pantel K, Brakenhoff RH: Dissecting the metastatic cascade. Nat Rev Cancer. 2004, 4: 448-456. 10.1038/nrc1370.View ArticlePubMedGoogle Scholar
- Pantel K, Brakenhoff RH, Brandt B: Detection, clinical relevance and specific biological properties of disseminating tumour cells. Nat Rev Cancer. 2008, 8: 329-340. 10.1038/nrc2375.View ArticlePubMedGoogle Scholar
- Yu M, Stott S, Toner M, Maheswaran S, Haber DA: Circulating tumor cells: approaches to isolation and characterization. J Cell Biol. 2011, 192: 373-382. 10.1083/jcb.201010021.View ArticlePubMedPubMed CentralGoogle Scholar
- Ashworth TR: A case of cancer in which cells similar to those in the tumors were seen in the blood after death. Aus Med J. 1869, 14: 146-147.Google Scholar
- Braun S, Pantel K, Muller P, Janni W, Hepp F, Kentenich CR, Gastroph S, Wischnik A, Dimpfl T, Kindermann G, Riethmuller G, Schlimok G: Cytokeratin-positive cells in the bone marrow and survival of patients with stage I, II, or III breast cancer. N Engl J Med. 2000, 342: 525-533. 10.1056/NEJM200002243420801.View ArticlePubMedGoogle Scholar
- Braun S, Vogl FD, Naume B, Janni W, Osborne MP, Coombes RC, Schlimok G, Diel IJ, Gerber B, Gebauer G, Pierga JY, Marth C, Oruzio D, Wiedswang G, Solomayer EF, Kundt G, Strobl B, Fehm T, Wong GY, Bliss J, Vincent-Salomon A, Pantel K: A pooled analysis of bone marrow micrometastasis in breast cancer. N Engl J Med. 2005, 353: 793-802. 10.1056/NEJMoa050434.View ArticlePubMedGoogle Scholar
- Cristofanilli M, Budd GT, Ellis MJ, Stopeck A, Matera J, Miller MC, Reuben JM, Doyle GV, Allard WJ, Terstappen LW, Hayes DF: Circulating tumor cells, disease progression, and survival in metastatic breast cancer. N Engl J Med. 2004, 351: 781-791. 10.1056/NEJMoa040766.View ArticlePubMedGoogle Scholar
- Nagrath S, Sequist LV, Maheswaran S, Bell DW, Irimia D, Ulkus L, Smith MR, Kwak EL, Digumarthy S, Muzikansky A, Ryan P, Balis UJ, Tompkins RG, Haber DA, Toner M: Isolation of rare circulating tumour cells in cancer patients by microchip technology. Nature. 2007, 450: 1235-1239. 10.1038/nature06385.View ArticlePubMedPubMed CentralGoogle Scholar
- Lianidou ES, Markou A: Circulating tumor cells in breast cancer: detection systems, molecular characterization, and future challenges. Clin Chem. 2011, 57: 1242-1255. 10.1373/clinchem.2011.165068.View ArticlePubMedGoogle Scholar
- Joosse SA, Pantel K: Biologic challenges in the detection of circulating tumor cells. Cancer Res. 2013, 73: 8-11. 10.1158/0008-5472.CAN-12-3422.View ArticlePubMedGoogle Scholar
- Lin HK, Zheng S, Williams AJ, Balic M, Groshen S, Scher HI, Fleisher M, Stadler W, Datar RH, Tai YC, Cote RJ: Portable filter-based microdevice for detection and characterization of circulating tumor cells. Clin Cancer Res. 2010, 16: 5011-5018. 10.1158/1078-0432.CCR-10-1105.View ArticlePubMedPubMed CentralGoogle Scholar
- Paterlini-Brechot P, Benali NL: Circulating tumor cells (CTC) detection: clinical impact and future directions. Cancer Lett. 2007, 253: 180-204. 10.1016/j.canlet.2006.12.014.View ArticlePubMedGoogle Scholar
- Ozkumur E, Shah AM, Ciciliano JC, Emmink BL, Miyamoto DT, Brachtel E, Yu M, Chen PI, Morgan B, Trautwein J, Kimura A, Sengupta S, Stott SL, Karabacak NM, Barber TA, Walsh JR, Smith K, Spuhler PS, Sullivan JP, Lee RJ, Ting DT, Luo X, Shaw AT, Bardia A, Sequist LV, Louis DN, Maheswaran S, Kapur R, Haber DA, Toner M: Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells. Sci Transl Med. 2013, 5: 179ra147-View ArticleGoogle Scholar
- Cristofanilli M, Hayes DF, Budd GT, Ellis MJ, Stopeck A, Reuben JM, Doyle GV, Matera J, Allard WJ, Miller MC, Fritsche HA, Hortobagyi GN, Terstappen LW: Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer. J Clin Oncol. 2005, 23: 1420-1430. 10.1200/JCO.2005.08.140.View ArticlePubMedGoogle Scholar
- Riethdorf S, Fritsche H, Muller V, Rau T, Schindlbeck C, Rack B, Janni W, Coith C, Beck K, Janicke F, Jackson S, Gornet T, Cristofanilli M, Pantel K: Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system. Clin Cancer Res. 2007, 13: 920-928. 10.1158/1078-0432.CCR-06-1695.View ArticlePubMedGoogle Scholar
- Allard WJ, Matera J, Miller MC, Repollet M, Connelly MC, Rao C, Tibbe AG, Uhr JW, Terstappen LW: Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with nonmalignant diseases. Clin Cancer Res. 2004, 10: 6897-6904. 10.1158/1078-0432.CCR-04-0378.View ArticlePubMedGoogle Scholar
- Pratt ED, Huang C, Hawkins BG, Gleghorn JP, Kirby BJ: Rare cell capture in microfluidic devices. Chem Eng Sci. 2011, 66: 1508-1522. 10.1016/j.ces.2010.09.012.View ArticlePubMedPubMed CentralGoogle Scholar
- Stott SL, Hsu CH, Tsukrov DI, Yu M, Miyamoto DT, Waltman BA, Rothenberg SM, Shah AM, Smas ME, Korir GK, Floyd FP, Gilman AJ, Lord JB, Winokur D, Springer S, Irimia D, Nagrath S, Sequist LV, Lee RJ, Isselbacher KJ, Maheswaran S, Haber DA, Toner M: Isolation of circulating tumor cells using a microvortex-generating herringbone-chip. Proc Natl Acad Sci USA. 2010, 107: 18392-18397. 10.1073/pnas.1012539107.View ArticlePubMedPubMed CentralGoogle Scholar
- Stott SL, Lee RJ, Nagrath S, Yu M, Miyamoto DT, Ulkus L, Inserra EJ, Ulman M, Springer S, Nakamura Z, Moore AL, Tsukrov DI, Kempner ME, Dahl DM, Wu CL, Iafrate AJ, Smith MR, Tompkins RG, Sequist LV, Toner M, Haber DA, Maheswaran S: Isolation and characterization of circulating tumor cells from patients with localized and metastatic prostate cancer. Sci Transl Med. 2010, 2: 25ra23-10.1126/scitranslmed.3000403.View ArticlePubMedPubMed CentralGoogle Scholar
- Issadore D, Chung J, Shao H, Liong M, Ghazani AA, Castro CM, Weissleder R, Lee H: Ultrasensitive clinical enumeration of rare cells ex vivo using a micro-hall detector. Sci Transl Med. 2012, 4: 141ra192-View ArticleGoogle Scholar
- Scheel C, Weinberg RA: Cancer stem cells and epithelial-mesenchymal transition: concepts and molecular links. Semin Cancer Biol. 2012, 22: 396-403. 10.1016/j.semcancer.2012.04.001.View ArticlePubMedGoogle Scholar
- Yu M, Bardia A, Wittner BS, Stott SL, Smas ME, Ting DT, Isakoff SJ, Ciciliano JC, Wells MN, Shah AM, Concannon KF, Donaldson MC, Sequist LV, Brachtel E, Sgroi D, Baselga J, Ramaswamy S, Toner M, Haber DA, Maheswaran S: Circulating breast tumor cells exhibit dynamic changes in epithelial and mesenchymal composition. Science. 2013, 339: 580-584. 10.1126/science.1228522.View ArticlePubMedPubMed CentralGoogle Scholar
- Alix-Panabieres C: EPISPOT assay: detection of viable DTCs/CTCs in solid tumor patients. Recent Results Cancer Res. 2012, 195: 69-76. 10.1007/978-3-642-28160-0_6.View ArticlePubMedGoogle Scholar
- Miyamoto DT, Lee RJ, Stott SL, Ting DT, Wittner BS, Ulman M, Smas ME, Lord JB, Brannigan BW, Trautwein J, Bander NH, Wu CL, Sequist LV, Smith MR, Ramaswamy S, Toner M, Maheswaran S, Haber DA: Androgen receptor signaling in circulating tumor cells as a marker of hormonally responsive prostate cancer. Cancer Discov. 2012, 2: 995-1003. 10.1158/2159-8290.CD-12-0222.View ArticlePubMedPubMed CentralGoogle Scholar
- Powell AA, Talasaz AH, Zhang H, Coram MA, Reddy A, Deng G, Telli ML, Advani RH, Carlson RW, Mollick JA, Sheth S, Kurian AW, Ford JM, Stockdale FE, Quake SR, Pease RF, Mindrinos MN, Bhanot G, Dairkee SH, Davis RW, Jeffrey SS: Single cell profiling of circulating tumor cells: transcriptional heterogeneity and diversity from breast cancer cell lines. PloS One. 2012, 7: e33788-10.1371/journal.pone.0033788.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang L, Ridgway LD, Wetzel MD, Ngo J, Yin W, Kumar D, Goodman JC, Groves MD, Marchetti D: The identification and characterization of breast cancer CTCs competent for brain metastasis. Sci Transl Med. 2013, 5: 180ra148-View ArticleGoogle Scholar
- Ameri K, Luong R, Zhang H, Powell AA, Montgomery KD, Espinosa I, Bouley DM, Harris AL, Jeffrey SS: Circulating tumour cells demonstrate an altered response to hypoxia and an aggressive phenotype. Br J Cancer. 2010, 102: 561-569. 10.1038/sj.bjc.6605491.View ArticlePubMedPubMed CentralGoogle Scholar
- Attard G, Swennenhuis JF, Olmos D, Reid AH, Vickers E, A'Hern R, Levink R, Coumans F, Moreira J, Riisnaes R, Oommen NB, Hawche G, Jameson C, Thompson E, Sipkema R, Carden CP, Parker C, Dearnaley D, Kaye SB, Cooper CS, Molina A, Cox ME, Terstappen LW, de Bono JS: Characterization of ERG, AR and PTEN gene status in circulating tumor cells from patients with castration-resistant prostate cancer. Cancer Res. 2009, 69: 2912-2918. 10.1158/0008-5472.CAN-08-3667.View ArticlePubMedGoogle Scholar
- Flores LM, Kindelberger DW, Ligon AH, Capelletti M, Fiorentino M, Loda M, Cibas ES, Janne PA, Krop IE: Improving the yield of circulating tumour cells facilitates molecular characterisation and recognition of discordant HER2 amplification in breast cancer. Br J Cancer. 2010, 102: 1495-1502. 10.1038/sj.bjc.6605676.View ArticlePubMedPubMed CentralGoogle Scholar
- Heitzer E, Auer M, Gasch C, Pichler M, Ulz P, Hoffmann EM, Lax S, Waldispuehl-Geigl J, Mauermann O, Lackner C, Hofler G, Eisner F, Sill H, Samonigg H, Pantel K, Riethdorf S, Bauernhofer T, Geigl JB, Speicher MR: Complex tumor genomes inferred from single circulating tumor cells by array-CGH and next-generation sequencing. Cancer Res. 2013, 73: 2965-2975. 10.1158/0008-5472.CAN-12-4140.View ArticlePubMedGoogle Scholar
- Magbanua MJ, Sosa EV, Roy R, Eisenbud LE, Scott JH, Olshen A, Pinkel D, Rugo HS, Park JW: Genomic profiling of isolated circulating tumor cells from metastatic breast cancer patients. Cancer Res. 2013, 73: 30-40. 10.1158/0008-5472.CAN-11-3017.View ArticlePubMedGoogle Scholar
- Magbanua MJ, Sosa EV, Scott JH, Simko J, Collins C, Pinkel D, Ryan CJ, Park JW: Isolation and genomic analysis of circulating tumor cells from castration resistant metastatic prostate cancer. BMC Cancer. 2012, 12: 78-10.1186/1471-2407-12-78.View ArticlePubMedPubMed CentralGoogle Scholar
- Hou Y, Song L, Zhu P, Zhang B, Tao Y, Xu X, Li F, Wu K, Liang J, Shao D, Wu H, Ye X, Ye C, Wu R, Jian M, Chen Y, Xie W, Zhang R, Chen L, Liu X, Yao X, Zheng H, Yu C, Li Q, Gong Z, Mao M, Yang X, Yang L, Li J, Wang W, et al: Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm. Cell. 2012, 148: 873-885. 10.1016/j.cell.2012.02.028.View ArticlePubMedGoogle Scholar
- Lu S, Zong C, Fan W, Yang M, Li J, Chapman AR, Zhu P, Hu X, Xu L, Yan L, Bai F, Qiao J, Tang F, Li R, Xie XS: Probing meiotic recombination and aneuploidy of single sperm cells by whole-genome sequencing. Science. 2012, 338: 1627-1630. 10.1126/science.1229112.View ArticlePubMedPubMed CentralGoogle Scholar
- Navin N, Kendall J, Troge J, Andrews P, Rodgers L, McIndoo J, Cook K, Stepansky A, Levy D, Esposito D, Muthuswamy L, Krasnitz A, McCombie WR, Hicks J, Wigler M: Tumour evolution inferred by single-cell sequencing. Nature. 2011, 472: 90-94. 10.1038/nature09807.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang J, Fan HC, Behr B, Quake SR: Genome-wide single-cell analysis of recombination activity and de novo mutation rates in human sperm. Cell. 2012, 150: 402-412. 10.1016/j.cell.2012.06.030.View ArticlePubMedPubMed CentralGoogle Scholar
- Xu X, Hou Y, Yin X, Bao L, Tang A, Song L, Li F, Tsang S, Wu K, Wu H, He W, Zeng L, Xing M, Wu R, Jiang H, Liu X, Cao D, Guo G, Hu X, Gui Y, Li Z, Xie W, Sun X, Shi M, Cai Z, Wang B, Zhong M, Li J, Lu Z, Gu N, et al: Single-cell exome sequencing reveals single-nucleotide mutation characteristics of a kidney tumor. Cell. 2012, 148: 886-895. 10.1016/j.cell.2012.02.025.View ArticlePubMedGoogle Scholar
- Zong C, Lu S, Chapman AR, Xie XS: Genome-wide detection of single-nucleotide and copy-number variations of a single human cell. Science. 2012, 338: 1622-1626. 10.1126/science.1229164.View ArticlePubMedPubMed CentralGoogle Scholar
- Auer M, Heitzer E, Ulz P, Geigl JB, Speicher MR: Single circulating tumor cell sequencing for monitoring. Oncotarget. 2013, 4: 812-813.View ArticlePubMedPubMed CentralGoogle Scholar
- Gangnus R, Langer S, Breit E, Pantel K, Speicher MR: Genomic profiling of viable and proliferative micrometastatic cells from early-stage breast cancer patients. Clin Cancer Res. 2004, 10: 3457-3464. 10.1158/1078-0432.CCR-03-0818.View ArticlePubMedGoogle Scholar
- Kraus J, Pantel K, Pinkel D, Albertson DG, Speicher MR: High-resolution genomic profiling of occult micrometastatic tumor cells. Genes Chromosomes Cancer. 2003, 36: 159-166. 10.1002/gcc.10160.View ArticlePubMedGoogle Scholar
- Solakoglu O, Maierhofer C, Lahr G, Breit E, Scheunemann P, Heumos I, Pichlmeier U, Schlimok G, Oberneder R, Kollermann MW, Kollermann J, Speicher MR, Pantel K: Heterogeneous proliferative potential of occult metastatic cells in bone marrow of patients with solid epithelial tumors. Proc Natl Acad Sci USA. 2002, 99: 2246-2251. 10.1073/pnas.042372199.View ArticlePubMedPubMed CentralGoogle Scholar
- Pantel K, Deneve E, Nocca D, Coffy A, Vendrell JP, Maudelonde T, Riethdorf S, Alix-Panabieres C: Circulating epithelial cells in patients with benign colon diseases. Clin Chem. 2012, 58: 936-940. 10.1373/clinchem.2011.175570.View ArticlePubMedGoogle Scholar
- Danila DC, Fleisher M, Scher HI: Circulating tumor cells as biomarkers in prostate cancer. Clin Cancer Res. 2011, 17: 3903-3912. 10.1158/1078-0432.CCR-10-2650.View ArticlePubMedPubMed CentralGoogle Scholar
- de Bono JS, Scher HI, Montgomery RB, Parker C, Miller MC, Tissing H, Doyle GV, Terstappen LW, Pienta KJ, Raghavan D: Circulating tumor cells predict survival benefit from treatment in metastatic castration-resistant prostate cancer. Clin Cancer Res. 2008, 14: 6302-6309. 10.1158/1078-0432.CCR-08-0872.View ArticlePubMedGoogle Scholar
- Olmos D, Arkenau HT, Ang JE, Ledaki I, Attard G, Carden CP, Reid AH, A'Hern R, Fong PC, Oomen NB, Molife R, Dearnaley D, Parker C, Terstappen LW, de Bono JS: Circulating tumour cell (CTC) counts as intermediate end points in castration-resistant prostate cancer (CRPC): a single-centre experience. Ann Oncol. 2009, 20: 27-33.View ArticlePubMedGoogle Scholar
- Kobayashi S, Boggon TJ, Dayaram T, Janne PA, Kocher O, Meyerson M, Johnson BE, Eck MJ, Tenen DG, Halmos B: EGFR mutation and resistance of non-small-cell lung cancer to gefitinib. N Engl J Med. 2005, 352: 786-792. 10.1056/NEJMoa044238.View ArticlePubMedGoogle Scholar
- Maheswaran S, Sequist LV, Nagrath S, Ulkus L, Brannigan B, Collura CV, Inserra E, Diederichs S, Iafrate AJ, Bell DW, Digumarthy S, Muzikansky A, Irimia D, Settleman J, Tompkins RG, Lynch TJ, Toner M, Haber DA: Detection of mutations in EGFR in circulating lung-cancer cells. N Engl J Med. 2008, 359: 366-377. 10.1056/NEJMoa0800668.View ArticlePubMedPubMed CentralGoogle Scholar
- Dickson MA, Shah MA, Rathkopf D, Tse A, Carvajal RD, Wu N, Lefkowitz RA, Gonen M, Cane LM, Dials HJ, Schwartz GK: A phase I clinical trial of FOLFIRI in combination with the pan-cyclin-dependent kinase (CDK) inhibitor flavopiridol. Cancer Chemother Pharmacol. 2010, 66: 1113-1121. 10.1007/s00280-010-1269-1.View ArticlePubMedPubMed CentralGoogle Scholar
- Ramaswamy B, Phelps MA, Baiocchi R, Bekaii-Saab T, Ni W, Lai JP, Wolfson A, Lustberg ME, Wei L, Wilkins D, Campbell A, Arbogast D, Doyle A, Byrd JC, Grever MR, Shah MH: A dose-finding, pharmacokinetic and pharmacodynamic study of a novel schedule of flavopiridol in patients with advanced solid tumors. Invest New Drugs. 2012, 30: 629-638. 10.1007/s10637-010-9563-7.View ArticlePubMedGoogle Scholar
- Wang LM, Ren DM: Flavopiridol, the first cyclin-dependent kinase inhibitor: recent advances in combination chemotherapy. Mini Rev Med Chem. 2010, 10: 1058-1070. 10.2174/1389557511009011058.View ArticlePubMedGoogle Scholar
- Bos PD, Nguyen DX, Massague J: Modeling metastasis in the mouse. Curr Opin Pharmacol. 2010, 10: 571-577. 10.1016/j.coph.2010.06.003.View ArticlePubMedPubMed CentralGoogle Scholar
- Comen E, Norton L, Massague J: Clinical implications of cancer self-seeding. Nat Rev Clin Oncol. 2011, 8: 369-377.PubMedGoogle Scholar
- Kim MY, Oskarsson T, Acharyya S, Nguyen DX, Zhang XH, Norton L, Massague J: Tumor self-seeding by circulating cancer cells. Cell. 2009, 139: 1315-1326. 10.1016/j.cell.2009.11.025.View ArticlePubMedPubMed CentralGoogle Scholar
- Yu M, Ting DT, Stott SL, Wittner BS, Ozsolak F, Paul S, Ciciliano JC, Smas ME, Winokur D, Gilman AJ, Ulman MJ, Xega K, Contino G, Alagesan B, Brannigan BW, Milos PM, Ryan DP, Sequist LV, Bardeesy N, Ramaswamy S, Toner M, Maheswaran S, Haber DA: RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis. Nature. 2012, 487: 510-513. 10.1038/nature11217.View ArticlePubMedPubMed CentralGoogle Scholar
- Baccelli I, Schneeweiss A, Riethdorf S, Stenzinger A, Schillert A, Vogel V, Klein C, Saini M, Bauerle T, Wallwiener M, Holland-Letz T, Hofner T, Sprick M, Scharpff M, Marme F, Sinn HP, Pantel K, Weichert W, Trumpp A: Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay. Nat Biotechnol. 2013, 31: 539-544. 10.1038/nbt.2576.View ArticlePubMedGoogle Scholar
- Chen XQ, Stroun M, Magnenat JL, Nicod LP, Kurt AM, Lyautey J, Lederrey C, Anker P: Microsatellite alterations in plasma DNA of small cell lung cancer patients. Nat Med. 1996, 2: 1033-1035. 10.1038/nm0996-1033.View ArticlePubMedGoogle Scholar
- Leon SA, Shapiro B, Sklaroff DM, Yaros MJ: Free DNA in the serum of cancer patients and the effect of therapy. Cancer Res. 1977, 37: 646-650.PubMedGoogle Scholar
- Nawroz H, Koch W, Anker P, Stroun M, Sidransky D: Microsatellite alterations in serum DNA of head and neck cancer patients. Nat Med. 1996, 2: 1035-1037. 10.1038/nm0996-1035.View ArticlePubMedGoogle Scholar
- Stroun M, Anker P, Maurice P, Lyautey J, Lederrey C, Beljanski M: Neoplastic characteristics of the DNA found in the plasma of cancer patients. Oncology. 1989, 46: 318-322. 10.1159/000226740.View ArticlePubMedGoogle Scholar
- Schwarzenbach H, Hoon DS, Pantel K: Cell-free nucleic acids as biomarkers in cancer patients. Nat Rev Cancer. 2011, 11: 426-437. 10.1038/nrc3066.View ArticlePubMedGoogle Scholar
- Leary RJ, Kinde I, Diehl F, Schmidt K, Clouser C, Duncan C, Antipova A, Lee C, McKernan K, De La Vega FM, Kinzler KW, Vogelstein B, Diaz LA, Velculescu VE: Development of personalized tumor biomarkers using massively parallel sequencing. Sci Transl Med. 2010, 2: 20ra14-10.1126/scitranslmed.3000702.View ArticlePubMedPubMed CentralGoogle Scholar
- McBride DJ, Orpana AK, Sotiriou C, Joensuu H, Stephens PJ, Mudie LJ, Hamalainen E, Stebbings LA, Andersson LC, Flanagan AM, Durbecq V, Ignatiadis M, Kallioniemi O, Heckman CA, Alitalo K, Edgren H, Futreal PA, Stratton MR, Campbell PJ: Use of cancer-specific genomic rearrangements to quantify disease burden in plasma from patients with solid tumors. Genes Chromosomes Cancer. 2010, 49: 1062-1069. 10.1002/gcc.20815.View ArticlePubMedPubMed CentralGoogle Scholar
- Diaz LA, Williams RT, Wu J, Kinde I, Hecht JR, Berlin J, Allen B, Bozic I, Reiter JG, Nowak MA, Kinzler KW, Oliner KS, Vogelstein B: The molecular evolution of acquired resistance to targeted EGFR blockade in colorectal cancers. Nature. 2012, 486: 537-540.PubMedPubMed CentralGoogle Scholar
- Diehl F, Li M, Dressman D, He Y, Shen D, Szabo S, Diaz LA, Goodman SN, David KA, Juhl H, Kinzler KW, Vogelstein B: Detection and quantification of mutations in the plasma of patients with colorectal tumors. Proc Natl Acad Sci UA. 2005, 102: 16368-16373. 10.1073/pnas.0507904102.View ArticleGoogle Scholar
- Diehl F, Schmidt K, Choti MA, Romans K, Goodman S, Li M, Thornton K, Agrawal N, Sokoll L, Szabo SA, Kinzler KW, Vogelstein B, Diaz LA: Circulating mutant DNA to assess tumor dynamics. Nat Med. 2008, 14: 985-990. 10.1038/nm.1789.View ArticlePubMedGoogle Scholar
- Forshew T, Murtaza M, Parkinson C, Gale D, Tsui DW, Kaper F, Dawson SJ, Piskorz AM, Jimenez-Linan M, Bentley D, Hadfield J, May AP, Caldas C, Brenton JD, Rosenfeld N: Noninvasive identification and monitoring of cancer mutations by targeted deep sequencing of plasma DNA. Sci Transl Med. 2012, 4: 136ra168-View ArticleGoogle Scholar
- Misale S, Yaeger R, Hobor S, Scala E, Janakiraman M, Liska D, Valtorta E, Schiavo R, Buscarino M, Siravegna G, Bencardino K, Cercek A, Chen CT, Veronese S, Zanon C, Sartore-Bianchi A, Gambacorta M, Gallicchio M, Vakiani E, Boscaro V, Medico E, Weiser M, Siena S, Di Nicolantonio F, Solit D, Bardelli A: Emergence of KRAS mutations and acquired resistance to anti-EGFR therapy in colorectal cancer. Nature. 2012, 486: 532-536.PubMedPubMed CentralGoogle Scholar
- Heitzer E, Auer M, Hoffmann EM, Pichler M, Gasch C, Ulz P, Lax S, Waldispuehl-Geigl J, Mauermann O, Mohan S, Pristauz G, Lackner C, Hofler G, Eisner F, Petru E, Sill H, Samonigg H, Pantel K, Riethdorf S, Bauernhofer T, Geigl JB, Speicher MR: Establishment of tumor-specific copy number alterations from plasma DNA of patients with cancer. Int J Cancer. 2013, 133: 346-356. 10.1002/ijc.28030.View ArticlePubMedPubMed CentralGoogle Scholar
- Chan KC, Jiang P, Zheng YW, Liao GJ, Sun H, Wong J, Siu SS, Chan WC, Chan SL, Chan AT, Lai PB, Chiu RW, Lo YM: Cancer genome scanning in plasma: detection of tumor-associated copy number aberrations, single-nucleotide variants, and tumoral heterogeneity by massively parallel sequencing. Clin Chem. 2013, 59: 211-224. 10.1373/clinchem.2012.196014.View ArticlePubMedGoogle Scholar
- Heitzer E, Ulz P, Belic J, Gutschi S, Quehenberger F, Fischereder K, Benezeder T, Auer M, Pischler C, Mannweiler S, Pichler M, Eisner F, Haeusler M, Riethdorf S, Pantel K, Samonigg H, Hoefler G, Augustin H, Geigl JB, Speicher MR: Tumor associated copy number changes in the circulation of patients with prostate cancer identified through whole-genome sequencing. Genome Med. 2013, 5: 30-10.1186/gm434.View ArticlePubMedPubMed CentralGoogle Scholar
- Leary RJ, Sausen M, Kinde I, Papadopoulos N, Carpten JD, Craig D, O'Shaughnessy J, Kinzler KW, Parmigiani G, Vogelstein B, Diaz LA, Velculescu VE: Detection of chromosomal alterations in the circulation of cancer patients with whole-genome sequencing. Sci Transl Med. 2012, 4: 162ra154-10.1126/scitranslmed.3004742.View ArticlePubMedPubMed CentralGoogle Scholar
- Murtaza M, Dawson SJ, Tsui DW, Gale D, Forshew T, Piskorz AM, Parkinson C, Chin SF, Kingsbury Z, Wong AS, Marass F, Humphray S, Hadfield J, Bentley D, Chin TM, Brenton JD, Caldas C, Rosenfeld N: Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA. Nature. 2013, 497: 108-112. 10.1038/nature12065.View ArticlePubMedGoogle Scholar
- Dawson SJ, Tsui DW, Murtaza M, Biggs H, Rueda OM, Chin SF, Dunning MJ, Gale D, Forshew T, Mahler-Araujo B, Rajan S, Humphray S, Becq J, Halsall D, Wallis M, Bentley D, Caldas C, Rosenfeld N: Analysis of circulating tumor DNA to monitor metastatic breast cancer. N Engl J Med. 2013, 368: 1199-1209. 10.1056/NEJMoa1213261.View ArticlePubMedGoogle Scholar
- Dawson SJ, Rosenfeld N, Caldas C: Circulating tumor DNA to monitor metastatic breast cancer. N Engl J Med. 2013, 369: 93-94.View ArticlePubMedGoogle Scholar
- Fabbri F, Carloni S, Zoli W, Ulivi P, Gallerani G, Fici P, Chiadini E, Passardi A, Frassineti GL, Ragazzini A, Amadori D: Detection and recovery of circulating colon cancer cells using a dielectrophoresis-based device: KRAS mutation status in pure CTCs. Cancer Lett. 2013, 335: 225-231. 10.1016/j.canlet.2013.02.015.View ArticlePubMedGoogle Scholar
- Peeters DJ, De Laere B, Van den Eynden GG, Van Laere SJ, Rothe F, Ignatiadis M, Sieuwerts AM, Lambrechts D, Rutten A, van Dam PA, Pauwels P, Peeters M, Vermeulen PB, Dirix LY: Semiautomated isolation and molecular characterisation of single or highly purified tumour cells from CellSearch enriched blood samples using dielectrophoretic cell sorting. Br J Cancer. 2013, 108: 1358-1367. 10.1038/bjc.2013.92.View ArticlePubMedPubMed CentralGoogle Scholar