Figure 2
From: Towards an integrated proteomic and glycomic approach to finding cancer biomarkers
Glycoproteomic flow chart reflecting the integration of traditional proteomics methods with glycomic techniques. This addresses the need to identify a glycoprotein (1), identify a glycosylation site (2), and determine the structure of the glycan (3). The ability to successfully characterize glycoproteins requires an emphasis on purification, enrichment, and fractionation strategies at both the protein and peptide level.