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Table 6 Validation of DKK1, UGT1A and EEF1A1 overexpression in the resistant cells

From: Networking of differentially expressed genes in human cancer cells resistant to methotrexate

  

Expression

Gene

Cell line

Microarray

RT-PCR validation

DKK1

HT29

4.25

5.66 ± 0.23

 

Caco-2

2.56

1.96 ± 0.03

UGT1A

MCF-7

15.90

23.41 ± 0.94

 

MDA-MB-468

19.27

16.28 ± 0.19

EEF1A1

MIA PaCa-2

2.29

3.89 ± 0.16

 

K562

2.75

2.38 ± 0.47

 

Saos-2

2.05

1.85 ± 0.15

  1. The expression levels for the three node genes (DKK1, UGT1A and EEF1A1) derived from both the microarray data (average of the overexpressed UGT1A family members) and real-time RT-PCR are given. All the results are expressed as fold changes relative to the sensitive cells and values are the mean of triplicate experiments ± SE.
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Genome Medicine

ISSN: 1756-994X

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