Longitudinal analysis of the peripheral B cell repertoire reveals unique effects of immunization with a new influenza virus strain

Table 1 Sequencing metrics of the analyzed V_H sequences

Sequencing results	Parameter	Totals^a	Amplicon library (Average ± SD)^b
Raw output	454 reads	1,792,504	40,738 ± 27,496
	Total bases (bp)	778,487,667	17,692,901 ± 12,073,169
	Average length (bp)	ND	483 ± 77
Quality and size filters	Eliminated reads (%)	27 %	24 % ± 14
Sequences included in the analysis	Analyzed with ImmunediveRsity	1,302,865	29,610 ± 18,114
	Clonotypes	71,568	1,626 ± 938
	Lineages	218,910	4,975 ± 3,378

^aTotal parameters of five 454-Roche Titanium sequencing runs. Forty-four gene libraries were generated from 13 individuals
^bEach gene library corresponds to one of six conditions: day 0 and day 7 after vaccination with TIV2008/2009, day 0 and day 7 after vaccination with 09 MIV2009, and day 0 and day 7 after vaccination with TIV2014/2015

ISSN: 1756-994X