Fig. 1

Cardiac innate immune response at steady state and after MI is different between C57BL/6 J and Rag2^del mice. A Schematic of experimental setup with 8 to 12-week-old male and female C57BL/6 J and Rag2^del mice. The sham group underwent two thoracotomies and an intramyocardial injection of Matrigel™. The MI group underwent two thoracotomies, permanent LAD ligation, and intramyocardial injection of Matrigel™. The MI-CM group underwent two thoracotomies, permanent LAD ligation, and intramyocardial injection of 1 million neonatal GFP cardiomyocytes suspended in Matrigel™. Flow cytometric analysis of the cardiac immune response at D4 and D7 after the initial thoracotomy (post OP) or LAD ligation (post MI) was carried out. B Differences in the percentage of cells at steady state between the sham groups. C Fold change (FC) difference of the percentages of various immune cells in the MI groups compared to the sham groups of respective C57BL/6 J and Rag2.^del mice. In both B and C, values are represented as mean ± SEM. N = 7 in all groups and time points. Significance was calculated using the Mann–Whitney test. *p ≤ 0.05