Fig. 4

Altered innate immune response following cardiomyocyte transplantation fails to improve cardiac function. A Representative tile scan of the heart 4 weeks after cell transplantation with an arrow pointing towards GFP signals observed at the injection site. Scale bar represents 400 μm. B Assessment of left ventricular ejection fraction (LVEF, %) and C end-systolic volume (ESV, μl) 4 weeks after MI/thoracotomy using MRI. D Changes in cardiac remodelling was monitored by assessing the left ventricular wall thickness from MRI images. E Strain analysis of the hearts was performed with the MRI Cine images using the feature-tracking strain analysis module and the peak mean circumferential strain was calculated. ‘MI’ refers to the infarct group, ‘MI-CM’ refers to the cell transplanted group, and ‘MI-CM + I’ refers to the cell transplantation combined with CCR2 inhibition. Values are represented as mean ± SEM. N = 7 in all groups. Significance was calculated separately for C57BL/6 J and Rag2^del mice using one-way ANOVA with Dunnett’s post hoc test for multiple comparisons. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001