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Fig. 4 | Genome Medicine

Fig. 4

From: Revealing the developmental origin and lineage predilection of neural progenitors within human bone marrow via single-cell analysis: implications for regenerative medicine

Fig. 4

Culture of BMSC-neurospheres with BMP4 agonist. A Culture conditions for BMSC-neurosphere generation and differentiation, with and without provision of BMP4 agonist SB4. B1 Immunocytochemistry revealed that BMSC-derived neurospheres expressed neural crest marker NGFR and neural markers Nestin, Tuj1 and S100β. B2 Quantitative real-time PCR results revealed that SB4 treatment led to a significant upregulation in neural differentiation-related genes BMP4, Pax6 and S100β, and corresponding downregulation in mesenchymal-related gene Dlx2. C1 After 10 days of culture in neural differentiation medium, differentiating cells were spindle-shaped and expressed both neuronal (Tuj1) and glial (S100β) markers. C2 SB4 treatment during differentiation resulted in significantly higher expression of glial cell marker S100β. N = 3; **, P < 0.01; *, P < 0.05; Unlabelled scale bars: 50 μm

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Genome Medicine

ISSN: 1756-994X

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