Fig. 2

Single-cell landscape of PitNET samples. a Matrix plot showing mean expression of known markers for each tumor sample. Samples are arranged by the classification of PitNETs. b UMAP plot showing the distribution of pseudo-bulk expression from all samples. c Boxplot showing pairwise similarities between pseudo-bulk expression profiles from different kinds of samples. The dashed line represents the mean correlation among all PitNET samples. ***P < 0.001, Student’s t test. d UMAP plot characterizing clusters of all cells from tumor samples. Upper left: UMAP plot labeled by the sample source. Upper right: UMAP plot labeled by all clusters. Lower left: UMAP plot of only epithelial cells in PIT1-positive samples. Lower right: UMAP plot of only epithelial cells in PIT1-negative samples. e The proportion of cells from four types of PitNETs for each cluster. f Bubble heatmap showing the characteristic marker genes of each cluster. g Boxplot showing the proportion of major cell types across different kinds of sample. *P < 0.05, **P < 0.01, Wilcoxon rank sum test. PIT1, PIT1-lineage PitNETs; SF1, SF1-lineage PitNETs; TPIT, TPIT-lineage PitNETs; NCT, null cell tumor