Fig. 5

Characterization of tumor-infiltrating immune cells and stromal cells in normal and PitNET samples. a UMAP plot showing clusters of immune cells and stromal cells from all samples. b Bubble heatmap showing the characteristic marker genes of each cluster. c Boxplots showing the changes of immune cell clusters between normal and tumor samples. d Inferred developmental trajectory of tumor-enriched myeloid cell types by RNA velocity. e Volcano plot showing differentially expressed genes between two tumor-enriched macrophage clusters. f Boxplots showing comparison of M1 and M2 phenotype signature across indicated macrophage clusters. Cluster filled in red has higher mean expression. Student’s t test. g Comparison of selected T cell function signatures between normal and tumor samples. h Boxplots showing the changes of stromal cell clusters between normal and tumor samples. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Student’s t test