Fig. 6

Aggressive tumor cell sub-populations. a The proportion of cells from each tumor sample for cluster T00. b Heatmap showing the highly expressed genes of cluster T00 in scRNA-seq data (left) and pan-cancer RNA-Seq data (right). MKI67 in blue serves as a selected differential marker for comparison, though it does not rank at the top. The fold change stands for tumors against adjacent normal tissues in TCGA. c The predicted aggressive degrees of the bulk pituitary tumor dataset by the random forest model. The left color panel stands for the clinical diagnosis of the samples. d Functional analyses by over-expressing and siRNA knockdown of GGH and PBK. The top panel stands for cell proliferation and the bottom panel for apoptosis. *P < 0.05, ***P < 0.001, ns, not significant, Student’s t test or one-way ANOVA. e, f Transwell migration analyses showed that PBK knockdown/overexpression would inhibited/promoted cell migration in GH3 cells, respectively. Left panel: representative images of transwell migration assay (scale bar: 100 μm). Right panel: quantitative analysis in different groups. **P < 0.01, ***P < 0.001. Student’s t test or one-way ANOVA. g Progression-free survival curves of PitNET cases with low (RNAscope counting <  = 50) and high (RNAscope counting > 50) PBK expression. Log-rank test