Fig. 2
From: De novo identification of expressed cancer somatic mutations from single-cell RNA sequencing data
Detecting expressed somatic mutations in the A375 cell line and pancreas tissue datasets. a Integrative Genomics Viewer (IGV) window shows the hotspot mutation BRAF V600E in the A375 cell line. b The scatter plot of the percentage of exonic SNVs validated in scRNA-seq against the million reads per cell. c The scatter plot shows the expression level against the number of expressed somatic mutations in each gene. d, e, The bar plot illustrates the distribution of VAF of expressed somatic mutations in scRNA-seq compared with corresponding VAF in bRNA-seq (d) and in WES (e). f The percentage of accumulated expressed SNVs validated in scRNA-seq. g The density plot shows the distribution of detected SNVs of each cell after passing the standard variant calling pipeline in the A375 cell line datasets with two conditions