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Table 4 List of MAPs selected for ex vivo immunoassay. Annotated list of 20 peptides identified in Mlh1-/- cells

From: Non-canonical antigens are the largest fraction of peptides presented by MHC class I in mismatch repair deficient murine colorectal cancer

Peptides

Genomic coordinates of pep seq

Region

#Reads

Alterations

Canonical

%AF

MS replicates

MS intensity

Gene FPKM

Pool

NKKNEGWLSKP

chr19:40559969-40560003

intron

10

WT

Non-canonical

100

1

NA

50.32

1

EMKPFIPI

chrX:104994035-104994060

extragenic

10

WT

Non-canonical

100

1

11750000

NA

1

FRGLTETTSSL

chr8:34821561-34821595

extragenic

12

WT

Non-canonical

100

1

NA

NA

1

FLRRCCLARYE

chrX:142351611-142351618 chrX:142390305-142390331

utr5

10

WT

Non-canonical

100

1

NA

26.06

1

LKIAWLREICL

chr9:14546274-14546308

utr3

16

SNV

Non-canonical

15.9574

1

NA

23.57

1

YRRFSFEG

chr7:118853174-118853202

cds

12

SNV

Non-canonical

14.4144

2

15288667

14.72

2

VKMPDAPR

chr12:105662622-105662648

cds

11

SNV

Non-canonical

18.5185

1

16421000

5.59

2

CASARCSPCLT

chr2:34870760-34870794

cds

15

SNV

Non-canonical

12.973

1

NA

39.25

2

AEKLKRAAEKD

chrX:136085159-136085193

utr5

12

WT

Non-canonical

100

1

NA

5.46

2

HKKPQPVPRAH

chr6:120482825-120482859

utr3

15

SNV

Non-canonical

15.8273

1

NA

9.42

2

RQWERTVKNKQ

chr5:67621541-67621575

utr3

11

WT

Non-canonical

100

1

NA

1.47

2

ELRLRHIAWAL

chr11:96712585-96712619

intron

14

WT

Non-canonical

100

1

NA

0.82

2

ITINPIKSMLF

chr16:30341231-30341265

cds

17

WT

Non-canonical

100

1

NA

43.64

3

IFPSIGDLALS

chr14:101918019-101918044 chr14:101918612-101918620

cds

14

WT

Non-canonical

100

1

NA

78.13

3

PSAFILIRKLG

chr7:45013466-45013500

cds

12

SNV

Non-canonical

16.7883

1

NA

38.55

3

PVVCKSQLAHG

chr1:164050176-164050187 chr1:164050644-164050666

cds

21

WT

Non-canonical

100

1

NA

20.21

3

VLVMFLLGKLP

chr4:82804025-82804058

utr3

18

WT

Non-canonical

100

1

NA

17.02

3

EPHSVTELKLE

chrX:136079187-136079221

intron

11

WT

Non-canonical

100

1

NA

5.46

3

SAVWTDLKMTP

chr1:153453947-153453981

extragenic

10

WT

Non-canonical

100

1

NA

NA

3

KCFSVISLYFE

chr5:67732187-67732221

cds

10

WT

Non-canonical

100

1

NA

1.47

3