Fig. 1

NGS-based spatial multi-omics. a Schematic of nondeterministic barcoding for spatial transcriptomics. Either barcoded spots (e.g., Visium) or beads (Slide-Seq) are used in an array to capture released reverse-transcribed RNA on a glass slide. Spatial multi-omic datasets can be generated using data integration with different single-cell methods. b Schematic of deterministic barcoding which utilizes PDMS chips with microchannels to barcode the tissue in two rounds. Several omic layers can be derived from the same tissue section including transcriptomics, proteomics, and epigenomics