Fig. 4

Mutation signatures in the pre-treatment tumors. Comparison of tumor mutation burden (A) and nucleotide substitutions (B) between the nonresponsive and responsive groups. C Distributions of the 10 main COSMIC signatures in the different NAC responsive groups across the 47 pre-treatment samples (left). Comparison of the relative weights of the signature 3 between the nonresponsive and responsive groups (right). D Heatmap comparison of the 22 genes statistically significantly related to the DNA repair pathway between the responsive and nonresponsive pre-treatment tumors. P values were calculated based on the Wilcoxon rank sum test. ***P < 0.001, **P < 0.01, *P < 0.05