Fig. 7

Identification of niches in human PDAC tissue. A Neighborhood graph representing neighborhood enrichment of cell types. Edges represent average neighborhood enrichment scores (observed-to-expected ratio) between the cell types, and only the bidirectional enrichments were depicted in this graph as edges. Dot sizes are proportional to the estimated abundances (log scale), and the colors represent average cancer cell abundances in each cell type’s neighborhood. B,C Representative images of deconvoluted spatial transcriptome data from two PDAC patients, colored with the abundances of three major cancer cell subclusters and two major fibroblast subclusters of PDAC. Orange dashed lines indicate cancer proximal niches, while blue dashed lines indicate cancer distal niches and the red dashed lines indicate putative cancer progression axis. D,E Average estimated abundances of the two major fibroblast subclusters, (D) Fb_LRRC15 and (E) Fb_SFRP1, in each epithelial subcluster’s neighborhood. F Correlation between the fraction of Fb_LRRC15 in the fibroblast population and the fraction of major cancer cell clusters in scRNA-seq data. Pearson’s r-value and p-value are denoted on the upper left corner of each plot