Fig. 5

Longitudinal CCC circuits during immune response to lentiviral pathogenic variants. A Diagram illustrating the definition of a longitudinal communicative circuit. B Stacked bar plot depicting the proportion of cell type annotations for cells from animal 170 A participating in longitudinal circuits. Proportions are separated by cells participating at the beginning or the end of the circuit, as well as the total distribution of all cells participating in circuits. C—D Scatter plots depicting ligand-target pairs participating in longitudinal communication circuits that differentiate 170-infected animals (y-axis) and CL8-infected animals (x-axis). For C, each point represents a ligand-target pair participating in a circuit at a particular time point. For D, each point represents the total number of mentions for each ligand-target pair in all circuits, regardless of time point. E Longitudinal circuits of pro-inflammatory cytokines in each animal. Circuits were restricted to pro-inflammatory cytokines (see Additional file 1: Table S5) and networks of connected pro-inflammatory cytokine ligand-target pairs plotted for each animal across time. Points are sized by the expression of the respective cytokine at each time point. Edges are colored by the product of the ligand activity score and the target weight for the sequential cytokine (see “Methods”)