Fig. 1

UDS-UMI/UMG variant calling is concordant with WES and mitigates FFPE-related sequencing artifacts. A UDS-UMI/UDG detected almost all mutations that were previously called in primary and metastatic tumors by WES. B Mutation VAFs were highly correlated between UDS-UMI/UDG and WES. C, D UDG treatment was effective in mitigating the majority of FFPE-related C>T artifacts from mutations called in rare subclones, as seen by the predominance of C>T rare subclonal mutations in untreated samples (n=5) compared with matched treated samples (n = 5) and with all treated samples (n = 30). C Considering trinucleotide context, a larger proportion of C>T covered sites exhibited rare subclonal mutations within untreated samples. D A larger proportion of rare subclonal mutations that were called within untreated samples occurred at C>T trinucleotide contexts. The 3’ (+1 position) nucleotide context of each mutation type is indicated by color