Fig. 1

The workflow of dMTLV and the performance evaluation based on simulation data. a The workflow of dMTLV. For read family containing candidate alleles, dMTLV first performs a likelihood test to remove alleles likely to be sequencing errors, then constructs consensus sequences containing both true variants and PCR errors. Based on this, dMTLV performs a second likelihood test to eliminate PCR errors and generates the final result. b The TPR, PPV, and F1 of four tools: MToolBox, Mutect2, VarScan2, and dMTLV in detecting 20 randomly simulated SNVs under seven distinct VAFs and three different coverages. The comparison of VAF from 0.001 to 0.01 was highlighted with rectangles in light pink. c The Pearson correlation between the simulated and the observed VAFs by each tool across three different coverages. All the correlations were significant, with a p-value less than 0.01