Fig. 5

CX3CR1⁺ macrophages interact with NR5A1⁺ tumor cells. A Interaction weights between TAM subpopulations and the three lineages of tumor cells. B Ligands and receptor pairs are involved in the interaction between TAM subpopulations and the three lineages of tumor cells. C Heatmap displaying the expression levels of ligands highly expressed in CX3CR1⁺ macrophages (left) and the expression levels of corresponding target genes on NR5A1⁺ tumor cells (right). D Signal pathways enriched from the ligand–receptor interaction between CX3CR1⁺ macrophages and NR5A1⁺ tumor cells. E Evaluation of the anti/pro-inflammatory and angiogenesis/phagocytosis functions of the three subtypes of macrophages. F Heatmap representing the mean expression levels of anti-tumor genes in the three subtypes of macrophages. G Violin plots illustrating the cell abundance of CX3CR1⁺ macrophages in samples categorized as low (< 3) or high (≥ 3) MIB1 percentage (on the left), as well as in samples with or without invasion. P-values were calculated using the Wilcoxon rank-sum test