Validation of miRNA targets. Three LCLs from non-autistic individuals were transfected with hsa-miR-29b pre-miR precursor, hsa-miR-219b anti-miR inhibitor, pre-miR negative control, or anti-miR negative control. At 72 hours after transfection, qRT-PCR analyses were conducted to determine expression of PLK2 and ID3 genes in the pre-miR/anti-miR-transfected LCLs (red), compared to respective pre-miR/anti-miR negative controls (navy). (a, b) Expression of PLK2 was significantly increased in the LCLs transfected with anti-miR-219-5p (a), whereas ID3 expression was significantly decreased in pre-miR-29b-transfected LCLs (b). The error bars show the standard error among the technical replicates. *P < 0.05.