Skip to main content

Advertisement

Table 2 Functional siRNA screening data for 11 GBM survival-associated genes in the A172 glioblastoma cell line

From: Large-scale data integration framework provides a comprehensive view on glioblastoma multiforme

Symbol Description siRNA name CTG Caspase Survival Expression
  AllStars Cell Death Vontrol siRNA (Cell death ctrl)   -13.80 19.62 NA NA
  AllStars Negative Control siRNA (siNEG)   0.00 0.00 NA NA
KIF11 Kinesin family member 11 KIF11_7 -9.56 7.39 NA NA
PLK1 Polo-like kinase 1 PLK1_7 -5.92 0.31 NA NA
FLNC Filamin C, gamma FLNC_2 2.56 -0.89 0.000189 2.51
   FLNC_5 -0.37 0.29   
   FLNC_6 -0.57 -0.94   
   FLNC_7 -5.39 0.49   
H19 H19, imprinted H19_1 0.38 -0.14 0.000588 3.54
  maternally expressed H19_2 -5.08 1.17   
  transcript (non-protein H19_3 5.20 -2.71   
  coding) H19_4 -2.97 2.71   
HIST1H4L Histone cluster 1, H4l HIST1H4L_1 1.63 -0.60 0.001560 5.01
   HIST1H4L_2 2.61 -2.00   
   HIST1H4L_5 -0.36 -1.19   
   HIST1H4L_7 -5.93 -0.97   
KIAA0040 KIAA0040 KIAA0040_11 -2.94 -0.64 0.000867 2.63
   KIAA0040_12 0.63 -0.83   
   LOC100129443_3 0.69 -0.71   
   LOC100129443_4 -0.31 -0.30   
LTF Lactotransferrin LTF_1 -1.74 0.22 0.001570 3.95
   LTF_2 -0.03 0.07   
   LTF_5 -1.04 0.44   
   LTF_6 -1.18 -0.79   
NNMT Nicotinamide N- NNMT_5 -0.91 -1.29 0.000074 7.41
  methyltransferase NNMT_6 -0.48 -1.87   
   NNMT_7 -1.26 -0.32   
   NNMT_8 -0.30 -0.10   
POSTN Periostin, osteoblast POSTN_1 0.24 -1.16 0.001950 14.9
  specific factor POSTN_2 0.28 -2.11   
   POSTN_6 -2.17 0.56   
   POSTN_7 0.52 0.12   
TAGLN2 Transgelin 2 TAGLN2_10 4.53 -1.74 0.001010 4.21
   TAGLN2_11 -3.78 0.48   
   TAGLN2_8 -0.43 0.45   
   TAGLN2_9 4.67 -1.94   
TIMP1 TIMP metallopeptidase TIMP1_2 -0.09 -0.96 0.000109 3.21
  inhibitor 1 TIMP1_4 1.07 0.88   
   TIMP1_5 0.56 -1.87   
   TIMP1_6 -0.08 0.41   
MSN Moesin MSN_8 -1.68 1.61 0.000028 3.42
   MSN_9 -4.49 -1.05   
   MSN_5 -0.36 -1.57   
   MSN_1 -2.57 0.80   
CDKN2A Cyclin-dependent kinase inhibitor 2A NA (gene deleted in A172)   
  1. Cell proliferation (CTG) and induction of caspase-3 and -7 activities (Caspase) were assayed after transfection of A172 cells with four siRNAs against each gene. Z-scores from the proliferation and caspase-3/7 assays are presented, centered on the scramble siRNA. Values in bold diverge by more than two standard deviation units from the median of scramble negative control siRNA and are considered significant. For each gene, the best survival P-value (Survival) and the corresponding fold change in the exon array (Expression) are given.