Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET

Table 3 Predicted and actual breakpoints from each sample

	Prediction		Break point	Actual		Difference (bp)
Sample	M-BCR	ABL1		M-BCR	ABL1	M-BCR	ABL1
K562	110,194-110,207	27,762-27,909	BCR-ABL1	110,191-110,192	27,878-27,879	3	0
KU812	110,241-110,242	63,843-63,853	BCR-ABL1	110,299-110,300	63,929-63,930	57	76
			ABL1-BCR	110,096-110,097	63,804-63,805	144	38
Patient 1	109,790-109,830	125,280-125,623	BCR-ABL1	109,781-109,782	125,326-125,327	8	0
			ABL1-BCR	109,670-109,671	149,445-149,446	119	^a23,822
Patient 2	109,702-109,867	102,484-102,653	BCR-ABL1	109,834-109,835	102,524-102,525	0	0
			ABL1-BCR	109,869-109,870	102,526-102,527	2	0

Predicted and actual breakpoints for each sample. The absolute difference (in base pairs) between predicted breakpoint site and sequenced breakpoint site is shown in the last two columns. All M-bcr coordinates are relative to chr22:23,522,552 (start position of BCR gene). All ABL1 coordinates are relative to chr9:133,586,268 (start position of ABL1 gene). ^aWe had a secondary peak at this locus in the patient 1 ABL1 breakpoint profile (Figure S3D in Additional file 1).

ISSN: 1756-994X