Skip to main content

Table 1 Summary of tumor heterogeneity studies

From: Future medical applications of single-cell sequencing in cancer

Cancer

Heterogeneity

Method

Details

Reference

Lung

Morphology

H&E staining

Microscopic examination

[67]

Pancreas

Morphology

H&E staining

Microscopic examination

[68]

Prostate

Morphology

H&E staining

Microscopic examination

[69]

Bladder

Morphology

H&E staining

Microscopic examination

[70]

Glioma

DNA

G-banding

G-banding and ploidy

[23]

Breast

DNA

G-banding

Karyotype G-banding

[25]

Breast

DNA

G-banding

Karyotype G-banding

[27]

Breast

DNA

G-banding

Karyotype G-banding

[94]

Bladder

DNA

FISH

DNA copy number analysis

[26]

Breast

DNA

FISH

DNA copy number analysis

[72]

Pancreas

DNA

FISH

DNA copy number analysis

[74]

Neuroblastoma

DNA

FISH

DNA copy number analysis

[73]

Breast

DNA

FISH

DNA copy number analysis

[28]

Multiple myeloma

DNA

FISH

DNA copy number analysis

[75]

Esophagus

DNA

FISH

FISH, LOH, microsatellites, sequencing

[76]

Breast

DNA

FISH

DNA copy number analysis

[71]

Breast (DCIS)

Protein

IHC

IHC using antibodies

[52]

Breast

Protein

MS

MS and LCM

[84]

Prostate

RNA

Expression

Transcriptional microarrays

[82]

Cervix

RNA

Expression

Transcriptional microarrays

[83]

Breast

DNA

CGH

LCM and BAC-CGH

[78]

Breast

DNA

CGH

Receptor-purification and SNP microarrays

[3]

Breast

DNA

CGH

Sectoring and aCGH

[2]

Breast

DNA

CGH

Sectoring, ploidy and aCGH

[1]

Cervix

DNA

CGH

Regional macrodissection and aCGH

[79]

Breast

DNA

NGS

NGS

[80]

Breast

DNA

NGS

NGS

[81]

Pancreas

DNA

NGS

Sectoring and NGS

[77]

Breast

DNA

NGS

Single-nucleus sequencing

[10]

  1. Summary of studies that have detected intratumor heterogeneity using various techniques, at the DNA, RNA and protein level. aCGH, microarray comparative genomic hybridization; BAC-CGH, bacterial artificial chromosome-comparative genomic hybridization; CGH, comparative genomic hybridization; DCIS, ductal carcinoma in situ; FISH, fluorescence in situ hybridization; H&E, hematoxylin and eosin; IHC, immunohistochemistry; LCM, laser-capture microdissection; LOH, loss of heterozygosity; MS, mass spectrometry; NGS, next-generation sequencing.