Skip to main content

Table 2 Transcriptomic comparisons between induced pluripotent stem cells and embryonic stem cells

From: Transcriptomic analysis of pluripotent stem cells: insights into health and disease

Characteristic Mouse iPSCs Human iPSCs
mRNA expression Distinct from mESCs at lower passages, donor cell gene expression still present [62, 69]; closely resemble mESCs at late passages [62] Distinct from hESCs at lower passages [58], with residual donor gene expression [70, 96, 97]; closely resemble hESCs at late passages [58, 98]
miRNA expression miRNA encoded within the imprinted Dlk1-Dio3 locus is aberrantly silenced [60] Small number of differences reported [58, 99], but variation between hESCs and hiPSCs comparable to somatic and cancer cells [100]
lncRNA expression Not determined Differences in lincRNA expression reported. lincRNA-RoR enhances reprogramming by twofold [13]
DNA methylation status Distinct from mESCs at lower passages, donor cell DNA methylation pattern still present [62, 69]; closely identical to mESCs at late passages [62] Differences in DNA methylation reported [59, 68, 70], but not in all hiPSCs [71]
Genome status Not determined Possess gene copy number deletions and duplications [63, 64], somatic coding mutations [65], and chromosomal duplications [66]
  1. hESC, human embryonic stem cell; hiPSC, human induced pluripotent stem cell; iPSC, induced pluripotent stem cell; lincRNA, long-intergenic non-coding RNA; lncRNA, long non-coding RNA; mESC, mouse embryonic stem cell; miRNA, microRNA.