Embryonic stem cell-specific signatures in cancer: insights into genomic regulatory networks and implications for medicine

Table 1 Genome-scale studies of self-renewal and pluripotency in ES cells

Regulators	Function	Methods	Reference(s)
Core factors
Oct4 (Pou5f1)	ES cell core factor	ChIP, MS	[27–32, 44]
Sox2	ES cell core factor	ChIP, MS	[28, 32, 44]
Nanog	ES cell core factor	ChIP, MS	[27–29, 32, 44]
Tcf3 (Tcf7l1)	Wnt signaling	ChIP	[74]
Klf4	LIF signaling	ChIP	[32, 44]
Stat3	LIF signaling	ChIP	[44]
Dax1 (Nr0b1)	Negative regulation of transcription	ChIP, MS	[29, 32]
Sall4	Self-renewal and pluripotency	ChIP, MS	[29, 75–77]
Polycomb-related factors
Ezh2	PRC2, repressor	ChIP, MS	[78, 79]
Jarid2	Fine-tuning of PRC2	ChIP, MS	[78, 79]
Mtf2	Polycomb-like protein	ChIP, MS	[78]
Suz12	PRC2, repressor	ChIP, MS	[37, 38, 78, 79]
Eed	PRC2, repressor	ChIP	[37, 38]
Rnf2	PRC1, repressor	ChIP	[37]
Phc1	PRC1, repressor	ChIP	[37]
Myc-related factors
Myc	Proliferation	ChIP, MS	[20, 44]
Max	Myc-interacting	ChIP, MS	[20]
Zfx	Self-renewal	ChIP	[44]
Trrap	Histone acetylation	RNAi	[50]
Tip60 (Kat5)	Histone acetylation	ChIP, MS, RNAi	[20, 50]
Ep400	Histone acetylation	MS, RNAi	[50]
Dmap1	Histone acetylation	ChIP, MS, RNAi	[20, 50]
E2F1	Regulator of cell cycle	ChIP	[44]
E2F4	Transcription activator	ChIP, MS	[20]
Cnot3	General transcription regulator	ChIP, RNAi	[80]
Trim28 (Tif1b)	Transcription co-activator	ChIP, RNAi	[80]

ISSN: 1756-994X