Figure 4
From: Metabolomics of human breast cancer: new approaches for tumor typing and biomarker discovery
Overview of the analysis of lipid metabolism in breast tumors. Using LC-MS, lipidomic profiles were measured in breast tumor, and these profiles were linked to analysis of key enzymes by immunohistochemistry (IHC) in corresponding tumor tissue samples. From an in silico analysis of candidate lipid pathways, siRNA knockdown experiments were designed to evaluate the function of these lipid-metabolizing enzymes for breast cancer proliferation and apoptosis. The detailed results of this part of the project have already been published [8].