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Table 1 Advances in the optimization of tissue culture conditions for clinical development of hESCs

From: Challenges to the clinical application of pluripotent stem cells: towards genomic and functional stability

Optimization of culture conditions of hESCs for clinical use References
Elimination of xeno-components Replacement of mouse feeder layer cells with human feeder layer cells [1821]
  Replacement of mouse feeder layer cells with human extracellular matrix [2225]
  hESC lines successfully banked under good laboratory practice conditions [1517]
Scaling up of production of hESCs Identification of small molecules that promote hESC self-renewal [2629]
  Synthetic microcarriers to support hESC culture in suspension [2932]