Figure 3
From: Mass spectrometry for translational proteomics: progress and clinical implications
Bottom-up MS approach. The most common MS-based proteomics approach is bottom-up analysis. In the bottom-up approach the proteins are first extracted from biofluids, cells or tissue. Enzymatic digestion of the proteins is then performed to fragment them into their corresponding peptide subunits, and the peptides are separated using LC and detected with MS. LC, liquid chromatography; MS, mass spectrometry; m/z, mass-to-charge ratio.