Figure 4

qRT-PCR validates Tag-seq results and identifies a robust gene set distinguishing GNS from NS cells. (a) Fold-change estimates (indicating expression level in GNS relative to NS cell lines) from Tag-seq and qRT-PCR for each of the 82 genes measured by qRT-PCR. Greater than two-fold difference in expression (dashed lines at y = ±1) was confirmed for all genes. (b) Heatmap of 29 genes differentially expressed between 16 GNS and six NS cell lines. Colors indicate qRT-PCR ΔΔC _t values, that is, normalized expression on a log₂ scale, where zero corresponds to the average expression between the two groups (GNS and NS cells). (c) Expression levels of the top six genes (by Wilcoxon test P-value) distinguishing GNS from NS cell lines, measured by qRT-PCR and presented as percentage of NS geometric mean.