Figure 3
From: Comprehensive analysis of the genome transcriptome and proteome landscapes of three tumor cell lines
Complex amplification of EGFR and PPARGC1A loci. (a) The region within the curly bracket is the tandemly duplicated unit in reverse orientation. It contains a 639 kb region (chr7: 54,973,500-55,632,000, red arrow, carrying the EGFR gene) and its inverted partial duplicate that contains the 1.3 Mb region on chr4 (chr4: 22,864,000-24-249,500, white box, carrying the PPARGC1A gene) and shorter regions from chromosomes 1, 21 and 3 (green, blue and purple boxes). (b) A431 cells in the metaphase, pink probes target amplified EGFR (340 kb), green probes target the centromere of chromosome 7. EGFR is located in chromosome 7 as well as in two minute chromosomes. (c) The probes targeting the PPARGC1A locus (chr4p15) and EGFR are visualized together, confirming the co-localization of these two heavily amplified loci in the minute chromosomes.