MicroRNA paraffin-based studies in osteosarcoma reveal reproducible independent prognostic profiles at 14q32

Table 3 Differential regulatory activity of prognostic miRNAs on the 14q32 locus

Differentially activated miRNA	Gene target prediction	Activity/enrichment assessment	P
hsa-miR-758	TargetScan	RE score	0.031
hsa-miR-299	TargetScan	RE score	0.034
hsa-miR-299-3p	TargetScan	RE score	0.034
hsa-miR-493	Pita	RE score	0.022
hsa-miR-323-5p	Pita	RE score	0.025
hsa-miR-411*	miRanda	GSA	0.005
hsa-miR-379*	miRanda	GSA	0.020
hsa-miR-139-5p	miRanda	GSA	0.005
hsa-miR-539	miRanda	GSA	0.047
hsa-miR-616*	miRanda	GSA	0.010
hsa-miR-493*	miRanda	GSA	0.025
hsa-miR-323-3p	miRanda	GSA	0.010
hsa-miR-382	miRanda	GSA	0.040

Using the regulatory effects scoring method with three different miRNA target prediction algorithms and miRanda-based GSA analysis, differentially activated miRNAs associated with recurrence were identified. Listed are those differentially activated miRNAs at 14q32 whose expression levels are associated with recurrence (P < 0.05). GSA, Gene Set Expression comparison Analyses; RE, Regulatory Effect

ISSN: 1756-994X