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Table 2 Measured levels of all fatty acids across 860 batches analyzed on three independent systems.

From: Development and validation of a robust automated analysis of plasma phospholipid fatty acids for metabolic phenotyping of large epidemiological studies

 

Fatty acid

Mix Std1a

QC1b

QC2c

  

Meand

SDe

CVf

Meand

SDe

CVf

Meand

SDe

CVf

C8:0

Caprylic

2.61

0.14

5.36

NDg

-

-

NDg

-

-

C10:0

Capric

3.72

0.15

4.12

0.01

BLOQh

-

0.01

BLOQh

-

C11:0

Undecanoic

2.01

0.07

3.50

ND

-

-

0.01

BLOQ

-

C12:0

Lauric

4.21

0.13

3.13

0.02

BLOQ

-

0.01

BLOQ

-

C13:0

Tridecanoic

2.19

0.06

2.82

0.02

BLOQ

-

0.11

BLOQ

-

C14:0

Myristic

4.41

0.10

2.35

0.25

0.04

15.90

0.18

0.04

21.60

C14:1

Myristoleic

2.16

0.06

2.69

ND

-

-

ND

-

-

C15:0

Pentadecanoic

2.23

0.05

2.06

0.16

0.03

16.07

0.13

BLOQ

-

C15:1

Pentadecenoic

2.16

0.05

2.35

ND

-

-

0.01

BLOQ

-

C16:0

Palmitic

6.65

0.19

2.89

30.12

0.43

1.43

15.02

0.35

2.30

C16:1

Palmitoleic

2.04

0.04

2.13

0.36

0.03

8.53

0.33

0.03

8.55

C17:0

Heptadecanoic

2.12

0.06

2.90

0.41

0.03

7.23

0.68

0.04

5.46

C17:1

Heptadecenoic

2.22

0.04

2.02

0.07

BLOQ

-

0.11

BLOQ

-

C18:0

Stearic

4.40

0.13

3.01

16.18

0.31

1.89

30.53

0.31

1.02

C18:1n9t

Trans-oleic

2.18

0.04

1.91

0.20

0.04

19.96

0.08

BLOQ

-

C18:1n9c

Cis-oleic

4.36

0.09

1.97

8.24

0.19

2.30

9.05

0.15

1.65

C18:2n6t

Trans-linoleic

2.10

0.05

2.26

0.06

BLOQ

-

0.35

0.07

19.11

C18:2n6c

Cis-linoleic

2.19

0.04

1.96

22.79

0.24

1.04

38.07

0.46

1.20

C18:3n6

γ-Linoleic

2.11

0.04

2.13

0.06

BLOQ

-

ND

-

-

C18:3n3

α-Linoleic

2.17

0.05

2.18

0.25

0.09

37.65

1.68

0.24

14.24

C20:0

Arachidic

4.39

0.15

3.38

0.14

0.02

17.80

0.70

0.07

9.69

C20:1

Eicosenoic

2.11

0.05

2.26

0.19

0.04

19.57

0.38

0.04

11.53

C20:2

Eicosadienoic

2.12

0.05

2.38

0.39

0.02

4.63

0.25

0.02

9.82

C20:3n6

Dihomo-γ-linoleic

2.08

0.06

2.95

3.45

0.09

2.67

0.41

0.05

12.66

C20:4n6

Arachidonic

4.02

0.09

2.22

11.15

0.19

1.67

0.98

0.05

5.57

C20:5n3

Eicosapentaenoic

2.13

0.09

4.26

0.47

0.03

7.20

0.20

0.04

18.48

C21:0

Heneicosanoic

2.17

0.08

3.70

ND

-

-

0.01

BLOQ

-

C22:0

Behenic

4.32

0.18

4.19

0.26

0.03

13.02

0.09

BLOQ

-

C22:1n9

Erucic

2.09

0.06

2.73

0.02

BLOQ

-

0.01

BLOQ

-

C22:2

Brassic

2.09

0.09

4.13

ND

-

-

0.02

BLOQ

-

C22:4

Adrenic

1.77

0.06

3.60

0.51

0.02

4.60

ND

-

-

C22:5n6

Osbond

0.00

0.00

0.00

0.32

0.03

9.24

0.01

BLOQ

-

C22:5n3

Docosapentaenoic

1.88

0.08

4.20

0.89

0.06

6.53

0.21

0.03

15.68

C22:6n3

Docosahexaenoic

1.79

0.05

2.90

2.20

0.09

4.05

0.11

BLOQ

-

C23:0

Tricosanoic

2.15

0.09

4.35

0.11

BLOQ

-

0.03

BLOQ

-

C24:0

Lignoceric

4.30

0.20

4.57

0.27

0.03

10.47

0.13

BLOQ

-

C24:1

Nervonic

2.13

0.07

3.16

0.36

0.04

12.31

0.17

0.03

18.32

  1. aMixture of fatty-acid methyl ester standards; fresh batches of mixtures were prepared every 1 to 2 months, and mean values were calculated for each batch (see Methods section).
  2. bNormal pooled human plasma (PLH-123-F; Sera Laboratories International).
  3. cNormal pooled horse plasma (S-121-F; Sera Laboratories International).
  4. dMean level determined over 860 batches using three independent systems.
  5. eStandard deviation determined over 860 batches using three independent systems.
  6. fCoefficient of variation (100% × SD/mean).
  7. gNot detected (level below 0.01%).
  8. hBelow limit of quantification (level below 0.15%). Mean values are given, but precision is insufficient.