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Table 2 Measured levels of all fatty acids across 860 batches analyzed on three independent systems.

From: Development and validation of a robust automated analysis of plasma phospholipid fatty acids for metabolic phenotyping of large epidemiological studies

  Fatty acid Mix Std1a QC1b QC2c
   Meand SDe CVf Meand SDe CVf Meand SDe CVf
C8:0 Caprylic 2.61 0.14 5.36 NDg - - NDg - -
C10:0 Capric 3.72 0.15 4.12 0.01 BLOQh - 0.01 BLOQh -
C11:0 Undecanoic 2.01 0.07 3.50 ND - - 0.01 BLOQ -
C12:0 Lauric 4.21 0.13 3.13 0.02 BLOQ - 0.01 BLOQ -
C13:0 Tridecanoic 2.19 0.06 2.82 0.02 BLOQ - 0.11 BLOQ -
C14:0 Myristic 4.41 0.10 2.35 0.25 0.04 15.90 0.18 0.04 21.60
C14:1 Myristoleic 2.16 0.06 2.69 ND - - ND - -
C15:0 Pentadecanoic 2.23 0.05 2.06 0.16 0.03 16.07 0.13 BLOQ -
C15:1 Pentadecenoic 2.16 0.05 2.35 ND - - 0.01 BLOQ -
C16:0 Palmitic 6.65 0.19 2.89 30.12 0.43 1.43 15.02 0.35 2.30
C16:1 Palmitoleic 2.04 0.04 2.13 0.36 0.03 8.53 0.33 0.03 8.55
C17:0 Heptadecanoic 2.12 0.06 2.90 0.41 0.03 7.23 0.68 0.04 5.46
C17:1 Heptadecenoic 2.22 0.04 2.02 0.07 BLOQ - 0.11 BLOQ -
C18:0 Stearic 4.40 0.13 3.01 16.18 0.31 1.89 30.53 0.31 1.02
C18:1n9t Trans-oleic 2.18 0.04 1.91 0.20 0.04 19.96 0.08 BLOQ -
C18:1n9c Cis-oleic 4.36 0.09 1.97 8.24 0.19 2.30 9.05 0.15 1.65
C18:2n6t Trans-linoleic 2.10 0.05 2.26 0.06 BLOQ - 0.35 0.07 19.11
C18:2n6c Cis-linoleic 2.19 0.04 1.96 22.79 0.24 1.04 38.07 0.46 1.20
C18:3n6 γ-Linoleic 2.11 0.04 2.13 0.06 BLOQ - ND - -
C18:3n3 α-Linoleic 2.17 0.05 2.18 0.25 0.09 37.65 1.68 0.24 14.24
C20:0 Arachidic 4.39 0.15 3.38 0.14 0.02 17.80 0.70 0.07 9.69
C20:1 Eicosenoic 2.11 0.05 2.26 0.19 0.04 19.57 0.38 0.04 11.53
C20:2 Eicosadienoic 2.12 0.05 2.38 0.39 0.02 4.63 0.25 0.02 9.82
C20:3n6 Dihomo-γ-linoleic 2.08 0.06 2.95 3.45 0.09 2.67 0.41 0.05 12.66
C20:4n6 Arachidonic 4.02 0.09 2.22 11.15 0.19 1.67 0.98 0.05 5.57
C20:5n3 Eicosapentaenoic 2.13 0.09 4.26 0.47 0.03 7.20 0.20 0.04 18.48
C21:0 Heneicosanoic 2.17 0.08 3.70 ND - - 0.01 BLOQ -
C22:0 Behenic 4.32 0.18 4.19 0.26 0.03 13.02 0.09 BLOQ -
C22:1n9 Erucic 2.09 0.06 2.73 0.02 BLOQ - 0.01 BLOQ -
C22:2 Brassic 2.09 0.09 4.13 ND - - 0.02 BLOQ -
C22:4 Adrenic 1.77 0.06 3.60 0.51 0.02 4.60 ND - -
C22:5n6 Osbond 0.00 0.00 0.00 0.32 0.03 9.24 0.01 BLOQ -
C22:5n3 Docosapentaenoic 1.88 0.08 4.20 0.89 0.06 6.53 0.21 0.03 15.68
C22:6n3 Docosahexaenoic 1.79 0.05 2.90 2.20 0.09 4.05 0.11 BLOQ -
C23:0 Tricosanoic 2.15 0.09 4.35 0.11 BLOQ - 0.03 BLOQ -
C24:0 Lignoceric 4.30 0.20 4.57 0.27 0.03 10.47 0.13 BLOQ -
C24:1 Nervonic 2.13 0.07 3.16 0.36 0.04 12.31 0.17 0.03 18.32
  1. aMixture of fatty-acid methyl ester standards; fresh batches of mixtures were prepared every 1 to 2 months, and mean values were calculated for each batch (see Methods section).
  2. bNormal pooled human plasma (PLH-123-F; Sera Laboratories International).
  3. cNormal pooled horse plasma (S-121-F; Sera Laboratories International).
  4. dMean level determined over 860 batches using three independent systems.
  5. eStandard deviation determined over 860 batches using three independent systems.
  6. fCoefficient of variation (100% × SD/mean).
  7. gNot detected (level below 0.01%).
  8. hBelow limit of quantification (level below 0.15%). Mean values are given, but precision is insufficient.