Skip to main content
Figure 2 | Genome Medicine

Figure 2

From: Circulating tumor cells and DNA as liquid biopsies

Figure 2

Workflow of CTC analyses. (a) CTCs (light blue cell) are rare cells in the circulation; the vast majority of nucleated cells are normal blood cells (orange). (b) First, separation steps as outlined in the main text are necessary to isolate these rare cells. (c) After cell lysis, DNA is accessible for whole-genome amplification (WGA). The WGA products can be analyzed for copy number changes on an array platform by comparative genomic hybridization (array CGH). Alternatively, libraries can be prepared and subjected to next-generation sequencing (NGS). By NGS both copy number changes and mutations within genes can be detected.

Back to article page