Multiplexed proteomics for co-measurement of cell migration and cytokine secretion of the same A549 (model lung carcinoma) cancer cells. (a) Light field images showing migration of three single cancer cells within microfluidic channels collected at 0 (before) and 24 (after) hours. (b) Heatmap: each column is a single-cell assay; each row is an assayed parameter. Cell migration distance (top row) is shown with the entire protein secretion profile (lower 14 rows). Approximately 1,000 single cells were assayed. (c) Scatter plots showing how the levels of three proteins (MCP-1 and IL-6) varied with cell migration distance. a.u., arbitrary units. Adapted from  with permission.