Coverage depth. (A) Fraction of bases with indicated coverage depth of genomic DNA (dark blue with mark, n = 1), amplified genomic DNA (red, n = 1), amplified DNA from multiple unfixed cells (yellow, n = 1), amplified DNA from multiple fixed cells (green, n = 2), and single unfixed (purple, n = 3) and fixed cells (blue, n = 4). (B) Cumulative fraction of the exome that was covered above coverage depths for the same samples as (A). The dotted lines indicate the standard deviations. The lines from 50 cells fixed (green) and 50 cells unfixed (yellow) mostly overlap. gDNA, genomic DNA; WGA, whole genome amplification.