Figure 8

Results of HpaII digestion of LTB4R followed by sequencing for promoter and gene body CpGs in human (Hs) and chimpanzee (Pt) (Martin et al .[19]via GEO). Methylation within the (A) promoter (Prom = CpG:99 in Figure 7) and (B) gene body (Body = CpG:76 in Figure 7) CpG islands of LTB4R from reverse scores (1 - p(U)) of all included HpaII MethylSeq sites analyzed by MetMap6 in purified neutrophils. These data replicate the significant difference identified in MeDIP peripheral blood. Four human and four chimpanzee samples had average methylation of 18.4% and 70.3% in the promoter (Prom), and 75.9% and 62.0% in the exonic gene body (Body) orthologous CpGis (Wilcoxon P < 0.05 for both).