Figure 1
Differential expression according to coronary artery disease status. (A,B) PC3 scores by disease classification in the two phases of transcriptome profiling. P-values from ANOVA with three degrees of freedom for group effect, implying replicated differential expression between individuals presenting with AMI and those with NO CAD, CAD and OLD MI, which are not consistently divergent. (C,D) Volcano plots of significance against difference in expression in log2 units of SNM-normalized data with genes upregulated in patients with AMI to the left, and genes downregulated in AMI to the right. Colored transcripts are significant at P < 10-5 in phase 2. Almost all of these are differentially expressed in the same direction in phase 1, which, however, showed much less evidence for significant differential expression in the total sample (possibly reflecting lower technical quality, or unknown covariates biasing against the AMI association). (E) Volcano plot of differential expression between non-AMI individuals who have had an MI on follow-up (n = 11) and the remainder, all in phase 1.