Figure 3

Validation of the BRCA1 -mutation DNAme signature in two independent prospective cohorts. ROC curves and AUC statistics to predict future breast cancer (BC) incidence applying the BRCA1-mutation DNAme signature in white blood cells (WBCs) (A) and in buccal (BUCC) cells (B) of the NSHD cohort and in serum DNA of the UKCTOCS cohort (C). Overlap of the top CpGs differently methylated in WBC between BRCA1 mutant and wild type (BRCA1 study) and the top CpGs differently methylated in serum DNA between women who have developed oestrogen receptor positive BCs and women who remained cancer-free (D). ROC curve and AUC statistics to predict deadly BCs applying the BRCA1-mutation DNAme signature in serum DNA in the UKCTOCS cohort (E) and Kaplan Meier curve (and hazard ratio (HR)) of future breast cancer patients with a high and low BRCA1-mutation DNAme score in serum DNA (F).