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Figure 4 | Genome Medicine

Figure 4

From: Development and validation of a new high-throughput method to investigate the clonality of HTLV-1-infected cells based on provirus integration sites

Figure 4

Validation of the tag system. For each control sample, both the expected and the experimentally observed patterns of raw sequence reads, shear sites, and the combination of tags and shear sites are represented in the bar graphs. Abbreviations: Com.: Combinations, Exp.: expected pattern, Seq.: raw sequencing data without removing PCR duplicates, Sh.: Shear sites, Tg.: Tags. (A) Clone size data of the first trial samples: Data were obtained considering the final optimal conditions: (Bowtie parameters: -v 3 - - best, and filtering condition: (merging approach) JT-10). (B) Clone size data of the second trial samples: Data were obtained considering the final optimal conditions: (Bowtie parameters: -v 3 - - best, and filtering condition: (merging approach) JT-10-1%). See Additional file 1: Figure S4 for information on merging approach.

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