Skip to main content

Table 2 Validation of dhfr overexpression and copy number determination in the different cell lines

From: Networking of differentially expressed genes in human cancer cells resistant to methotrexate

 

Expression

  

Cell line

Microarray

RT-PCR validation

Copy number

Protein

HT29

7.1

10.8 ± 0.7

16.1 ± 1.4

++

Caco-2

46.7

49.7 ± 1.1

83.4 ± 8.1

ND

MCF-7

31.1

33.2 ± 0.7

58.1 ± 0.8

+++++

MDA-MB-468

1.8

3.4 ± 0.1

0.9 ± 0.1

ND

MIA PaCa-2

9.5

8.2 ± 1.1

32.2 ± 2.2

+++

K562

9.4

9.8 ± 0.2

1.9 ± 0.1

++++

Saos-2

4.1

4.1 ± 1.1

0.6 ± 0.1

+

  1. The overexpression of DHFR was validated at the mRNA and protein levels by real-time RT-PCR and western blotting using a specific antibody, respectively. DHFR expression levels are presented both as the values found in the microarrays and as validated by RT-PCR. dhfr copy number was determined by real-time PCR. Values are the mean (in fold change relative to the sensitive cells) of three independent experiments ± SE. Protein levels were qualitatively assessed (+ to +++++) from the western blot images. ND, non-determined.