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Table 2 Validation of dhfr overexpression and copy number determination in the different cell lines

From: Networking of differentially expressed genes in human cancer cells resistant to methotrexate

  Expression   
Cell line Microarray RT-PCR validation Copy number Protein
HT29 7.1 10.8 ± 0.7 16.1 ± 1.4 ++
Caco-2 46.7 49.7 ± 1.1 83.4 ± 8.1 ND
MCF-7 31.1 33.2 ± 0.7 58.1 ± 0.8 +++++
MDA-MB-468 1.8 3.4 ± 0.1 0.9 ± 0.1 ND
MIA PaCa-2 9.5 8.2 ± 1.1 32.2 ± 2.2 +++
K562 9.4 9.8 ± 0.2 1.9 ± 0.1 ++++
Saos-2 4.1 4.1 ± 1.1 0.6 ± 0.1 +
  1. The overexpression of DHFR was validated at the mRNA and protein levels by real-time RT-PCR and western blotting using a specific antibody, respectively. DHFR expression levels are presented both as the values found in the microarrays and as validated by RT-PCR. dhfr copy number was determined by real-time PCR. Values are the mean (in fold change relative to the sensitive cells) of three independent experiments ± SE. Protein levels were qualitatively assessed (+ to +++++) from the western blot images. ND, non-determined.