Figure 4

Generation and biological evaluation of peptide binders against predicted drug targets. (A,B) Peptide targets PPWD1-WD40 domain (A) and NXF1-LRR domain (B) are selected based on the known domain-peptide structures (top and left) and genomic/network topological properties and probability score (top and right). Structures of WD40 domain (Protein Data Bank (PDB) ID: 1NEX) and LRR domain (PDB ID: 3P72) are shown. High-affinity peptide binders against PPWD1-WD and NXF1-LRR and their sequence motifs are shown (bottom and left). Cell viabilities depending on the infection efficiency of lentiviruses are compared (bottom and right). Both peptides show decrease in cell viability in a dose-dependent manner as opposed to GFP control (Additional file 13). MOI represents multiplicity of infection. *P-value <0.1.