Figure 1From: VISPA: a computational pipeline for the identification and analysis of genomic vector integration sitesIS analysis procedure for the Roche 454 sequencing platform. (A) DNA fragments containing the vector-cellular genome junctions are retrieved and amplified from vector marked genomic DNA by LAM-PCR. (B) LAM-PCR products are processed by the NGS platform, yielding sequencing reads that have to be processed in silico. (C) Bioinformatics pipeline, from FASTA extraction to IS identification and annotation.Back to article page