Overview of the Capture-C approach. Capture-C  enables mapping of chromatin interactions, in this example between a regulatory element (within the region denoted by a red line) and a gene promoter (gray line). Crosslinking and high-efficiency restriction digestion followed by proximity ligation (in which close proximity will favor ligation taking place, in this example generating red-gray lines in contrast to black lines representing other ligation events) allows such interactions to be defined. A 3C library is generated, sonicated and end repair performed with ligation of adaptors (dark gray boxes). Capture of target regions of interest (in this example target is region denoted by red line) involves oligonucleotide capture technology (capture probes denoted by red hexagons with yellow centers). Sequencing using end-ligated adapters allows genome-wide sites of interaction to be revealed. The approach can be multiplexed to several hundred targets.