Figure 2

Unsupervised analysis highlights transcriptional relationships between antigen-presenting cell populations. (a) Correlation matrix of samples obtained from the 27,935 transcripts detected in the dataset (Illumina detection P-values <0.01 in at least 1 of 80 samples). (b) Hierarchical clustering (Pearson correlation) of the 9,955 transcripts differentially expressed between the eight populations studied. (c) Histogram representing the weighted average proportion variance for tissue, cell population and the interactive term of these two parameters measured by PVCA. (d) Principal component analysis of the 80 samples analyzed, classified according to the normalized expression of the 9,955 transcripts identified above. The samples are colored either by tissue (left panel) or cell population (right panel). Both three-dimensional (upper panels) and two-dimensional PCA are displayed (lower panels). (e) Heatmap representing the results of Tukey’s test. The values plotted represent the number of transcripts differentially expressed between each pair of cell populations.