The nCounter® platform has a large dynamic range and can be used with RNA extracted from various malaria patient sample types. (A, B) Linearity of total transcript counts versus absolute number of in vitro culture-adapted 3D7 parasites that were synchronized and isolated as (A) rings, or (B) schizonts. Horizontal lines indicate mean transcript counts among all genes. (C, D) Processed sample transcript counts versus pre-processing lysate transcript counts for (C) a mock filter paper sample, and (D) a mock formalin fixed paraffin embedded (FFPE) sample. (C) Culture-adapted 3D7 parasites were synchronized and grown to ring stage, and then were spotted onto Whatman filter paper. (D) Culture-adapted 3D7 parasites were synchronized and grown to trophozoite stage, and then parasitized red blood cells were clotted and fixed in formalin before paraffin embedding, sectioning, and processing.