P. falciparum transcriptional profiles can be measured in vitro and in vivo with nCounter® using 328 genes. (A) In vitro intraerythrocytic gene expression measured in two different 3D7 ring-stage and schizont-stage cultures. Spearman correlations between nCounter® expression and in vitro expression , is plotted versus life cycle time point. Representative illustrations of parasite developmental stages are included from . (B-E) In vivo sequestered parasite gene expression measured in postmortem blood and tissues from Malawian children that succumbed to cerebral malaria (B-D), or another cause of death (E). Spearman correlations between nCounter® expression and in vitro expression are plotted versus life cycle time point. Numbers in parentheses in the legend of each panel are the number of parasites counted in 10 high-power fields by microscopy.