Figure 3
From: Comparison of DNA methylation profiles in human fetal and adult red blood cell progenitors
Examples of loci differentially methylated between fetal- and adult stage erythroblasts. Each vertical line represents a targeted CpG. Red and blue lines correspond to CpGs significantly hypomethylated in fetal- and adult-stage erythroblasts, respectively (P <1.25 × 10−7 and Δβ ≥0.2). (A) A CpG in the promoter of GCNT2 isoform C, which is responsible for the conversion of the fetal i-antigen to the adult I-antigen, is hypomethylated in adult-stage erythroblasts. (B) GCNT2-C expression levels is inversely correlated with DNA methylation at a CpG located in its core promoter (r = −0.97, P = 4.6 × 10−14). (C) CpGs in an intron of ARID3A, a gene implicated in hematopoiesis, displays hypomethylation in adult-stage erythroblasts. (D) DNA methylation inside ARID3A (highlighted) positively correlates with its expression levels (r = 0.87, P = 9.6 × 10−8). (E) Differential DNA methylation near C22orf26 and the miRNA let7-b host gene (MIRLET7BHG). A cluster of hypomethylated CpGs in adult erythroblasts overlaps an active erythroid enhancer (purple horizontal bars). DNA methylation inside the enhancer (highlighted) negatively correlates with (F) MIRLET7BHG (r = −0.99, P = 5.6 × 10−20) and (G) C22orf26 expression levels (r = −0.74, P = 3.2 × 10−5).