miRNA-protein associations validated by cell line functional assessment. Individual miRNAs were overexpressed in the MDA-MB-231 breast cancer cell line and the effect on protein expression was assessed. Shown are miRNA-protein associations that were both estimated based on the Oslo2 patient data and confirmed with the in vitro cell line experiment. The numbers in the white boxes represent protein cluster number in cases where several proteins share miRNA associations. Red lines indicate positive associations and blue lines negative associations. Yellow nodes represent miRNAs and blue nodes represent genes/proteins. The figure was made using Cytoscape version 2.8.3 .