A long non-coding RNA is highly activated in colorectal tumors. (a) Expression of EVADR in tumor and adjacent normal tissue from 65 patients with colorectal carcinoma. For each subject (x-axis) tumor is shown in red and normal tissue in grey. The dashed line indicates an arbitrary minimum expression threshold of 10 FPKM. A total of 21 patients demonstrated robust (>10 FPKM) levels of EVADR expression. Normal colon tissue was generally not found to express EVADR or it was expressed at low levels (<10 FPKM). (b) Schematic representation of the chromosomal location of the EVADR gene locus. Arrowheads indicate the orientation of transcription. Bar plots indicate mean expression levels for respective genes across all 65 tumor (COL91A, 0.25 ± 1.11 FPKM; EVADR, 14.4 ± 24.5 FPKM; FAM135A, 7.2 ± 3 FPKM (mean ± SD)) and normal samples (COL91A, 0.19 ± 0.53 FPKM; EVADR, 0.50 ± 1.52 FPKM; FAM135A, 5 ± 2.6 FPKM (mean ± SD)). *P < 0.00001; paired t-test. (c) Exon structure and primer locations for the lncRNA EVADR. (d) Representative gel image showing EVADR transcript levels in colorectal tumor (T32) and matched normal (N32) tissue samples for patient 32, measured by RT-PCR. The letter L indicates the molecular ladder.